Protein Info for PFLU_RS29900 in Pseudomonas fluorescens SBW25-INTG

Annotation: DNA helicase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 PF00580: UvrD-helicase" amino acids 12 to 274 (263 residues), 297.2 bits, see alignment E=2.9e-92 PF13245: AAA_19" amino acids 16 to 257 (242 residues), 78.6 bits, see alignment E=1e-25 PF13361: UvrD_C" amino acids 279 to 618 (340 residues), 273.2 bits, see alignment E=8.4e-85 PF13538: UvrD_C_2" amino acids 558 to 614 (57 residues), 29.8 bits, see alignment 8.5e-11

Best Hits

Swiss-Prot: 66% identical to UVRD_ECOLI: DNA helicase II (uvrD) from Escherichia coli (strain K12)

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 100% identity to pfs:PFLU6075)

MetaCyc: 66% identical to DNA helicase II (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4G1 at UniProt or InterPro

Protein Sequence (727 amino acids)

>PFLU_RS29900 DNA helicase II (Pseudomonas fluorescens SBW25-INTG)
MRDDLSLLLNSLNDAQRQAVAAPVGRQLVLAGAGSGKTRVLVHRIAWLIQVENASPHSIL
SVTFTNKAAAEMRHRIEQLMGISPAGMWVGTFHGLAHRLLRAHWQEAGLSQTFQILDSDD
QQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGLRPQHIQASGDLFLATMRSIYEAYE
AACVRAGVIDFSELLLRALDLWRDNPGLLAHYQKRFRHILVDEFQDTNAVQYAWLRLLAK
GGDSLMVVGDDDQSIYGWRGAKIENIYQYSEDFPDAVTIRLEQNYRSTAGILKAANALIA
NNTGRLGKELWTDGGEGEAINLYAAFNEHDEARYVVETIESALKTGLARSDIAILYRSNA
QSRVLEEALLRERIPYRIYGGQRFFERAEIKNAMAYLRLLEGRGNDAALERVINIPARGI
GEKTVEAIRDHARHADVSMWEAMRLLIANKGLTGRAAGALGVFVELIENLAAKCAEMPLH
LMTQTVIEQSGLIAYHEAEKGEKGQARVENLEELVSAARAFENTEEDEELTPLAAFLGHA
SLEAGDTQADEHEDSIQLMTLHSAKGLEFPYVFLVGMEEGLFPHKMSLEEPGRLEEERRL
AYVGITRAMQNLVMTYAETRRLYGSETYNKVSRFVREVPKGLIQEVRLSNSVSRPFGGGQ
QQNSSSMFAGSEIPETQFSLGQQVRHSVFGEGVILNFEGAGAQARVQVNFAEGSKWLMMG
YAKLEAI