Protein Info for PFLU_RS29550 in Pseudomonas fluorescens SBW25-INTG

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 PF17191: RecG_wedge" amino acids 12 to 171 (160 residues), 66.3 bits, see alignment E=7.4e-22 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 27 to 663 (637 residues), 749.5 bits, see alignment E=1.9e-229 PF01336: tRNA_anti-codon" amino acids 61 to 132 (72 residues), 29.5 bits, see alignment E=1.7e-10 PF04851: ResIII" amino acids 273 to 429 (157 residues), 37.9 bits, see alignment E=5.3e-13 PF00270: DEAD" amino acids 289 to 434 (146 residues), 88.6 bits, see alignment E=1.2e-28 PF00271: Helicase_C" amino acids 480 to 585 (106 residues), 66.6 bits, see alignment E=6.8e-22 PF19833: RecG_dom3_C" amino acids 614 to 676 (63 residues), 54.9 bits, see alignment E=2.4e-18

Best Hits

Swiss-Prot: 59% identical to RECG_ECO57: ATP-dependent DNA helicase RecG (recG) from Escherichia coli O157:H7

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to pfs:PFLU6003)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K491 at UniProt or InterPro

Protein Sequence (691 amino acids)

>PFLU_RS29550 ATP-dependent DNA helicase RecG (Pseudomonas fluorescens SBW25-INTG)
MTELSQVSVTALKGVGEAMAEKLAKVGLENLQDVLFHLPLRYQDRTRVVPIGQLRPGQDA
VVEGTVSGADVVMGKRRSLVVRLQDGTGGLSLRFYHFSNAQKEGLKRGTRVRCYGEARPG
ASGLEIYHPEYRAITGDEPPPVDTTLTPIYPLTEGLTQQRLRQLCMQTLTMLGPKSLPDW
LPEELARDYQLAPLDDAIRYLHHPPADADVDELALGHHWAQHRLAFEELLTHQLSQQRLR
ESMRSLRAPAMPKATRLPAQYLANLGFAPTGAQQRVGNEIAYDLSQKEPMLRLIQGDVGA
GKTVVAALAALQALEAGYQVALMAPTEILAEQHFITFKRWLEPLGLEVAWLAGKLKGKNR
ASALEQIAGGAPMVVGTHALFQDEVQFKNLALVIIDEQHRFGVQQRLALRQKGVGGRMNP
HQLIMTATPIPRTLAMSAYADLDTSILDELPPGRTPVNTVLVTDTRRVEVIERVRGACAE
GRQAYWVCTLIEESEELTCQAAETTYEDLTSALGELKVGLIHGRMKPAEKAAVMAEFKAG
NLQLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGSAASHCVLLYHPPL
SQIGRQRLGIMRETNDGFVIAEKDLELRGPGEMLGTRQTGLLQFKVADLMRDADLLPAVR
DAAQALLERWPEHVSPLLNRWLRHGQQYGQV