Protein Info for PFLU_RS29340 in Pseudomonas fluorescens SBW25-INTG

Annotation: bifunctional diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 554 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 120 to 274 (155 residues), 82.3 bits, see alignment E=1.6e-27 PF00990: GGDEF" amino acids 125 to 274 (150 residues), 107 bits, see alignment E=8.6e-35 PF00563: EAL" amino acids 298 to 533 (236 residues), 260 bits, see alignment E=1.9e-81

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5960)

Predicted SEED Role

"GGDEF domain/EAL domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K450 at UniProt or InterPro

Protein Sequence (554 amino acids)

>PFLU_RS29340 bifunctional diguanylate cyclase/phosphodiesterase (Pseudomonas fluorescens SBW25-INTG)
MSTPVEPLRLLLLADEPAWAALLRECLAPMGDGAVLISAPNWESVSRLFDDDHSAVLLTT
PSLQPGPGRCSLPCVLLLEQEPLVSPLGVSDWLVRDVLDADTLRRCLRHVRERGLLENTL
QRLAEQDPLTGIANRQGFQTLLAARLAENDGRGLALGHLDLDNFRHANDALGHQAGDRLI
LQVVSRLKSQLEAGDQLARLGSDEFALLIDTRRAPQRAEWMAERITEVMAEPYWVDGESL
LIGCSLGVAHARARAGADPLMWHAHIAMQQAKSTQGCTFHIFNERINRNARSLADLESEL
RRALRRDELELHYQPRLDLDDGHIVGLEALVRWRHGERGLLPPSEFVPLAEQSGLIVPLG
YWVISRALRDMQDLRERGLPPLHMAVNLSFRQFQDSQLLSTLSRLIAERGVEAQWLEFEL
TETAVMRRSDLVKQTMDALGRLGVRFSLDDFGTGFSSFVHLNSLPIALLKIDKSFVGGME
EREENRKLVHAMINLAHNLNLEVVAEGVETPEQLALLRLFGCDQAQGYLISKPLPLPELV
DYLTFGKSQQALLG