Protein Info for PFLU_RS29240 in Pseudomonas fluorescens SBW25-INTG

Annotation: class I adenylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 946 PF12633: Adenyl_cycl_N" amino acids 20 to 216 (197 residues), 257.2 bits, see alignment E=1.3e-80 PF01295: Adenylate_cycl" amino acids 244 to 797 (554 residues), 394.7 bits, see alignment E=8.2e-122

Best Hits

KEGG orthology group: K05851, adenylate cyclase, class 1 [EC: 4.6.1.1] (inferred from 100% identity to pfs:PFLU5940)

Predicted SEED Role

"Adenylate cyclase (EC 4.6.1.1)" in subsystem cAMP signaling in bacteria (EC 4.6.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.6.1.1

Use Curated BLAST to search for 4.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K430 at UniProt or InterPro

Protein Sequence (946 amino acids)

>PFLU_RS29240 class I adenylate cyclase (Pseudomonas fluorescens SBW25-INTG)
MTRTHEIRPDLDEGIDRKVLAQLRARFMALNEGRMARAVEGLTPRQQSVLTLLPLFFHVN
HPLLPGYVSGSTPAGLSNFEPDTQALAEAQRLTRSFSYKPRHGNPPRPIHGLFLMGSLGT
LAQADQSDMDVWVCHAADLSESELAELRKKCQLLEAWALTMGAEAHFFLIEPTRFVLGER
DTQLSSDDCGTTQHYLLLDEFYRTAIWLAGRTPIWWLVPVYEETHYAEFTHTLISKRFIR
ADETLDLGHLARIPPGEFIGAGLWQLFKGIESPYKSVLKLLLTEVYASEHPNVHCLSLRF
KRAVFANQMDLDELDPYIVVYRRIEEYLKARHEPERLELVRRALYLKVNRKLSAGQRTTG
WQRLLLERLAHEWGWDQRQLTLLDSRSQWKVRQVASERRALVSELNYSYRFLTQFARTEQ
TVSLINKRDLNVLGRRLYAAFERKAGKVEFINPGIAPDLAEDTLTLVQSPNRKEPGQHHW
GLYNGNLTALEWEHFAPIKRSRDLLEMLTWCHRNGVIDSSTRLALHPGTSDMTEFELFNL
LGSLQQTIALPLASVDEDRLLRSAVPEEVLLLINVGVDPLKHHRDLNILMTTERTDSLSY
AGVRDNLVLTLDQVTLNSWNEVMVSRYDGPHALLDCLRDYLNQLPPDHLPRLRVRCFCHN
RAQFIAQRVEEIFDTAQNLLLGQGNHRYLLQVQQHYHVMELMPGQATHVSLPTQDALIAY
LSEELASYSPLHLDAMALEDHDLALLLPMGQADSVQVFYRVNEGFAELYVLDEFNALWQQ
RLPFHDEQSLLAPLQRFLQSIIYRRDALSPLDPQEPLGAVHILYYQLLPAGSGRARGIEP
RPAPLNPANKPFYDVQAIIGKAAPGQVGITLYCNQREFSELEFGDQLFAVVAQEIVGQRR
ETERYRCYITDLDLSGLLGDVQSPSNLYLRYKAELELSLNEALSQI