Protein Info for PFLU_RS29215 in Pseudomonas fluorescens SBW25-INTG

Annotation: response regulator transcription factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 PF00072: Response_reg" amino acids 3 to 113 (111 residues), 81.9 bits, see alignment E=3.8e-27 PF04397: LytTR" amino acids 149 to 245 (97 residues), 74.8 bits, see alignment E=5.5e-25

Best Hits

Swiss-Prot: 80% identical to ALGR_PSEAE: Positive alginate biosynthesis regulatory protein (algR) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K08083, two-component system, LytT family, response regulator AlgR (inferred from 100% identity to pfs:PFLU5935)

Predicted SEED Role

"Autolysis response regulater LytR" in subsystem Murein hydrolase regulation and cell death

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K425 at UniProt or InterPro

Protein Sequence (248 amino acids)

>PFLU_RS29215 response regulator transcription factor (Pseudomonas fluorescens SBW25-INTG)
MNVLIVDDEPQARERLSRLVSELEGYAVLEPSATTGEEALTLIDSLKPDVVLLDIRMPGL
DGLQVAARLSERESPPAVVFCAAGEEFSAETLEASGVSFLTKPVAGETLLKALKKAERPT
RVQLAALTQPAAQSGNGPRSHISARTRKGIELIPLGEVVYFIADHKYVTLRHQGGEVLLD
EPLKALEDEFGDRFVRIHRNALVARERIERLQRTPLGHFQLFLKGLNGDALIVSRRHVAG
VRKMMQQL