Protein Info for PFLU_RS29065 in Pseudomonas fluorescens SBW25-INTG

Annotation: amino acid ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 243 PF00005: ABC_tran" amino acids 19 to 166 (148 residues), 132.7 bits, see alignment E=3e-42 PF13401: AAA_22" amino acids 28 to 194 (167 residues), 29.6 bits, see alignment E=1.6e-10

Best Hits

Swiss-Prot: 54% identical to GLUA_CORGL: Glutamate transport ATP-binding protein GluA (gluA) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)

KEGG orthology group: K02028, polar amino acid transport system ATP-binding protein [EC: 3.6.3.21] (inferred from 100% identity to pfs:PFLU5906)

MetaCyc: 51% identical to L-glutamine ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-12-RXN [EC: 7.4.2.1]

Predicted SEED Role

"Glutamate transport ATP-binding protein"

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.21

Use Curated BLAST to search for 3.6.3.21 or 7.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3Z6 at UniProt or InterPro

Protein Sequence (243 amino acids)

>PFLU_RS29065 amino acid ABC transporter ATP-binding protein (Pseudomonas fluorescens SBW25-INTG)
MPLLRISALHKYYGDHHVLKGIDLTVEEGQVVAIIGRSGSGKSTLLRTLNGLESINDGVI
EVDGEYLDAARADLRSLRQKVGMVFQQFNLFPHLTVGENVMLAPQVVQKVPKAKAAQLAR
QMLERVGLGEKFDAFPDRLSGGQQQRVAIARALAMSPKVLLCDEITSALDPELVNEVLSV
VRQLAKDGMTLIMVTHEMRFAREVGDKLVFMHQGKVHEVGDPKQLFANPQTVELANFIGS
TEQ