Protein Info for PFLU_RS28525 in Pseudomonas fluorescens SBW25-INTG

Annotation: class I SAM-dependent rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF17785: PUA_3
  • amino acids 6 to 72 (67 residues), 50.6 bits, E=6.3e-17
• PF02475: Met_10
  • amino acids 120 to 288 (169 residues), 39.2 bits, E=2.9e-13
• PF10672: Methyltrans_SAM
  • amino acids 173 to 345 (173 residues), 65.2 bits, E=2.6e-21
• PF03602: Cons_hypoth95
  • amino acids 215 to 303 (89 residues), 42.5 bits, E=2.6e-14
• PF05175: MTS
  • amino acids 220 to 335 (116 residues), 36.1 bits, E=2.3e-12
• PF13847: Methyltransf_31
  • amino acids 220 to 341 (122 residues), 33.8 bits, E=1.3e-11
• PF09445: Methyltransf_15
  • amino acids 222 to 346 (125 residues), 39.5 bits, E=2e-13
• PF01170: UPF0020
  • amino acids 244 to 342 (99 residues), 24.5 bits, E=9.2e-09

Best Hits

Swiss-Prot: 36% identical to RLMI_ERWT9: Ribosomal RNA large subunit methyltransferase I (rlmI) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K06969, ribosomal RNA large subunit methyltransferase I [EC: 2.1.1.-] (inferred from 100% identity to pfs:PFLU5795)

Predicted SEED Role

"LSU m5C1962 methyltransferase RlmI" in subsystem Ribosome biogenesis bacterial

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

See C3K3N8 at UniProt or InterPro

Protein Sequence (398 amino acids)

>PFLU_RS28525 class I SAM-dependent rRNA methyltransferase (Pseudomonas fluorescens SBW25-INTG)
MSLPSLRLKANADRRLRNGHLWVYSNEIDVAATPLHGFQAGDQAILEAAGGKTLGIVAMS
PNNLICARLLSRDIKLPLDKSLLVHRLNVALSLRDRLFDKPFYRLVYGDSDLLPGLVVDR
FGDILVVQIASATMEAHKEDVIAALTQVLKPSGILFKNDSAARDAEGLNRYVETVFGLVP
EWVALEENGVKFEAPVIQGQKTGWFYDHRMNRARLAPYAKGKRVLDLYSYIGGWGVQAAA
FGASEVFCVDASAFALDGVERNAALNGVAEKMTCIEGDVFEALKELKASEERFDVIVADP
PAFIKRKKDMKNGEGAYRRLNEQAMRLLSKDGILVSASCSMHLPEDDLQNILLTSARHLD
RNIQMLERGGQGPDHPVHPAIVETRYIKSITCRLLPNS