Protein Info for PFLU_RS28465 in Pseudomonas fluorescens SBW25-INTG

Annotation: insulinase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 transmembrane" amino acids 9 to 28 (20 residues), see Phobius details PF00675: Peptidase_M16" amino acids 86 to 224 (139 residues), 24.1 bits, see alignment E=3e-09 PF05193: Peptidase_M16_C" amino acids 230 to 406 (177 residues), 118 bits, see alignment E=4.5e-38

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5783)

Predicted SEED Role

"FIG015287: Zinc protease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3M6 at UniProt or InterPro

Protein Sequence (496 amino acids)

>PFLU_RS28465 insulinase family protein (Pseudomonas fluorescens SBW25-INTG)
MSDRKSSRLILPGLIVVTLIAAGAVYFLRPNESVASQALEKAQSSNQLQSLAELDGKAPT
NRKLDVQTWTTAEGAKVLFVEAHELPMFDVRILFAAGSSQDGNVPGLALMTNAMLNEGVP
GKDVSQIARDFEGLGADFGNGAYRDMALVSLRSLSDSDKRDAALALFDDVIGKPTFPADS
LARIKNQILAGFEYQKQNPAKLASLELFKRLYGDHPYAHPTEGTPESVPKITLAQLQAFH
AKAYAAGNAVIAVVGDLTRAEAEAMTAKVSASLPKGPALAKIAQPTEPKPGLSRIEFPSK
QTHLLFAQLGIDRADPDYAALSLGNQILGGGGFGTRLMSEVREKRGLTYGVYSGFSPMQV
RGPFMINLQTRAEMSGGTLRLVEDVVADYLKTGPTQKELDDAKRELAGSFPLSTASNADI
VGQLGAMGFYNLPLSYLEDFMKQSQALTVDQVKAAMNKHLSADKMVIVTAGPTIAQKPLP
PPTDKPAEQPLGVPEH