Protein Info for PFLU_RS28460 in Pseudomonas fluorescens SBW25-INTG

Annotation: insulinase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00675: Peptidase_M16" amino acids 40 to 167 (128 residues), 71.5 bits, see alignment E=7.4e-24 PF05193: Peptidase_M16_C" amino acids 196 to 378 (183 residues), 116.1 bits, see alignment E=1.8e-37

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5782)

Predicted SEED Role

"FIG015547: peptidase, M16 family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3M5 at UniProt or InterPro

Protein Sequence (451 amino acids)

>PFLU_RS28460 insulinase family protein (Pseudomonas fluorescens SBW25-INTG)
MNALARRAAGLLFSTVCLPLSALAADPQPTHEFTLDNGLKVVVREDHRAPVVVSQVWYKV
GSSYETPGQTGLSHALEHMMFKGSAKVGPGEASLILRDLGAEENAFTSDDYTAYYQVLAR
DRLGVAFELEADRMASLRLPADEFSREIEVIKEERRLRTDDNPMSKAYERFKAMAFPASG
YHTPTIGWMADLDRMKVEELRHWYQSWYVPNNATLVVVGDVTPDEVKNLAQRYFGPIPKR
DVPPAKIPMELAEPGERQLTLHVQTQLPSVMLGFNVPGLATADDKRSVQALRLISALLDG
GYSARISEQLERGEELVSAASTNYDAYTRGDTLFMLSATPNQQKKKTVAQAEAGLWRLLD
ELKAKPPTAEELERIRAQVIAGLVYQRDSITSQATAIGSLETVGLSWKLMDTELADLQSV
TPEDIQKAARTYFTRERLSVAHVLPQETAHE