Protein Info for PFLU_RS28415 in Pseudomonas fluorescens SBW25-INTG

Annotation: tRNA (guanosine(46)-N7)-methyltransferase TrmB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 TIGR00091: tRNA (guanine-N(7)-)-methyltransferase" amino acids 54 to 240 (187 residues), 199.3 bits, see alignment E=2e-63 PF02390: Methyltransf_4" amino acids 66 to 235 (170 residues), 186.9 bits, see alignment E=1.1e-59

Best Hits

Swiss-Prot: 100% identical to TRMB_PSEFS: tRNA (guanine-N(7)-)-methyltransferase (trmB) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03439, tRNA (guanine-N7-)-methyltransferase [EC: 2.1.1.33] (inferred from 100% identity to pfs:PFLU5772)

MetaCyc: 62% identical to tRNA m7G46 methyltransferase (Escherichia coli K-12 substr. MG1655)
tRNA (guanine-N(7)-)-methyltransferase. [EC: 2.1.1.33]

Predicted SEED Role

"tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)" (EC 2.1.1.33)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3L6 at UniProt or InterPro

Protein Sequence (241 amino acids)

>PFLU_RS28415 tRNA (guanosine(46)-N7)-methyltransferase TrmB (Pseudomonas fluorescens SBW25-INTG)
MTESNETPNTPEAGDESKHRRIKSFVMRAGRMTEGQQKGLEQGTPLFVLPLADAPVDYDQ
VFGRSAPRSLEIGFGMGHSLLEMAAAAPDQDFIGVEVHRPGVGALLNGVLTQGLTNLRVY
DCDAIEVLNRCIADNSLDRLMLFFPDPWHKARHHKRRIVQASFAELVRSKLKVGGILHMA
TDWEPYAEYMLEVMNVAPGYRNLAEDGKCVPRPAERPITKFERRGERLGHGVWDLKFEKL
A