Protein Info for PFLU_RS28385 in Pseudomonas fluorescens SBW25-INTG

Annotation: homoserine O-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to METXS_PSEFS: Homoserine O-succinyltransferase (metXS) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00641, homoserine O-acetyltransferase [EC: 2.3.1.31] (inferred from 100% identity to pfs:PFLU5766)

Predicted SEED Role

"Homoserine O-acetyltransferase (EC 2.3.1.31)" in subsystem Methionine Biosynthesis (EC 2.3.1.31)

MetaCyc Pathways

superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
L-methionine biosynthesis III (4/4 steps found)
superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
L-homocysteine biosynthesis (2/2 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.31

Use Curated BLAST to search for 2.3.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3L0 at UniProt or InterPro

Protein Sequence (379 amino acids)

>PFLU_RS28385 homoserine O-acetyltransferase (Pseudomonas fluorescens SBW25-INTG)
MPTAFPPDSVGLVVPQMAHFSEPLALACGRSLPAYDLIYETYGQLNATASNAVLICHALS
GHHHAAGFHSVDERKPGWWDSCIGPGKPIDTNKFFVVSLNNLGGCNGSTGPSSLNPENGK
PFGADFPVLTVEDWVHSQARLADLLGIDQWAAVIGGSLGGMQALQWTITYPDRVRHCLAI
ASAPKLSAQNIAFNEVARQAILTDPEFHGGSFQEAGVIPKRGLMLARMVGHITYLSDDSM
GEKFGRGLKSEKLNYDFHSVEFQVESYLRYQGEEFSGRFDANTYLLMTKALDYFDPAANF
DDDLAKTFEGATAKFCVMSFTTDWRFSPARSRELVDALMAARKDVCYLEIDAPQGHDAFL
IPIPRYLQAFSNYMNRITL