Protein Info for PFLU_RS28275 in Pseudomonas fluorescens SBW25-INTG

Annotation: malonate decarboxylase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 PF16957: Mal_decarbox_Al" amino acids 11 to 556 (546 residues), 979.7 bits, see alignment E=1.7e-299 TIGR01110: malonate decarboxylase, alpha subunit" amino acids 11 to 556 (546 residues), 1025.3 bits, see alignment E=0

Best Hits

KEGG orthology group: K13929, malonate decarboxylase alpha subunit [EC: 2.3.1.187] (inferred from 100% identity to pfs:PFLU5743)

MetaCyc: 78% identical to malonate decarboxylase acetyl-[acp]:malonate-[acp] transferase component (Klebsiella pneumoniae)
Biotin-independent malonate decarboxylase. [EC: 4.1.1.88]; Acetyl-S-ACP:malonate ACP transferase. [EC: 4.1.1.88, 2.3.1.187]

Predicted SEED Role

"Malonate decarboxylase alpha subunit" in subsystem Malonate decarboxylase

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.88

Use Curated BLAST to search for 2.3.1.187 or 4.1.1.88

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3I9 at UniProt or InterPro

Protein Sequence (556 amino acids)

>PFLU_RS28275 malonate decarboxylase subunit alpha (Pseudomonas fluorescens SBW25-INTG)
MTTTITPDSRWTRRRDEKQRRLGLVKKYADGAVLPSDRIVEALEALILPGDRVVLEGNNQ
KQADFLSRSLAKADPAKLHDLHMIMPSVGRSEHLDLFEKGIARKLDFSFAGTQSLRISQL
LEDGLLEIGAIHTYIELYARLVVDLIPNVVLSAGFMADRAGNIYTGASTEDTPALIEPAA
FSDGIVIVQVNQLVDDVTDLPRVDIPASWVDFVVVADKPFYIEPLFTRDPRHIKPVHVLM
AMMAIRGIYEKHNVQSLNHGIGFNTAAIELILPTYGESLGLKGKICRNWTLNPHPTLIPA
IESGWVQSVHCFGTELGMENYIAARPDVFFTGRDGSMRSNRMFCQLAGQYAVDLFIGATL
QVDGDGHSSTVTRGRLAGFGGAPNMGHDPRGRRHGTPAWLDMRHDDAPEALLERGKKLVV
QMVETFQEGGKPTFVDTLDAVEVARKSGMPLAPIMIYGDDVTHLLTEEGIAYLYKARSLE
ERQAMIAAVAGVTAIGMRHNPKDTARMRREGLIALPEDLGIRRTDATRELLAAKSVADLV
EWSGGLYNPPAKFRSW