Protein Info for PFLU_RS27845 in Pseudomonas fluorescens SBW25-INTG

Annotation: NAD(P)-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 PF00890: FAD_binding_2" amino acids 56 to 90 (35 residues), 22.7 bits, see alignment (E = 1e-08) PF13450: NAD_binding_8" amino acids 58 to 129 (72 residues), 44 bits, see alignment E=4.5e-15 PF01593: Amino_oxidase" amino acids 63 to 577 (515 residues), 124.9 bits, see alignment E=1.1e-39

Best Hits

KEGG orthology group: K00466, tryptophan 2-monooxygenase [EC: 1.13.12.3] (inferred from 100% identity to pfs:PFLU5656)

Predicted SEED Role

"Lysine 2-monooxygenase (EC 1.13.12.2)" in subsystem Lysine degradation (EC 1.13.12.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.13.12.2, 1.13.12.3

Use Curated BLAST to search for 1.13.12.2 or 1.13.12.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3A3 at UniProt or InterPro

Protein Sequence (605 amino acids)

>PFLU_RS27845 NAD(P)-binding protein (Pseudomonas fluorescens SBW25-INTG)
MSAIPSRSKGAARLLTNDVTALIPDFPFHYAKFLNSAVSNGKPLAGVSTRQQGTQVLIVG
AGVAGLVAAYEAMRMGLHPVVVEASDRIGGRFYSYVAGDPQDSANSVICEMGAMRFPVSG
KALMHYFDKVGMTANSADFPNPGSAATPSTVVDYQQRQVYYEGKNLPPEYEDIESKLFDV
FLEQDPIKFLEMETAMSGDNPDLAKIKTLWNAILDKGWDNLSFFAAMVEEAGWSREDIDL
FGQIGFGTGGWNTDYPNCFLEVLRVLYTGLDTNHKLMYDGSSELPTRLWNRAPDALGDPM
AYWPAGTTVQGLTAQVIAQPFNQEVRQIIRRPDGEFDVWMFDHNRGSERQYTFAAVVYTP
HKRVLDKFRFMDGVARFDQMNSLLSIKEWEAVTYTHYMQSAKIFAQTQRPFWTDVGADRQ
YKMSVTLSDRITRGTYLLDYAASQGPYKGSGMFLSYTWNDDSIKFLGDRAAPLPSHVAVC
STLLDSVYAHHQLNLAAEFGMTDPFVEINWEEQPFFLSAFKMNLPGQYEYQRLLFSQFMN
GVFDGSPDGFILAGDDVSFTGGWGEGAVTTALNAVNNLAVKFGGGTFIENRGPIDDWQVL
KPKAL