Protein Info for PFLU_RS27500 in Pseudomonas fluorescens SBW25-INTG

Annotation: multifunctional CCA addition/repair protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01743: PolyA_pol
  • amino acids 4 to 122 (119 residues), 63.1 bits, E=5e-21
• PF12627: PolyA_pol_RNAbd
  • amino acids 149 to 212 (64 residues), 45.4 bits, E=8.7e-16
• PF01966: HD
  • amino acids 230 to 311 (82 residues), 35.6 bits, E=1.5e-12

Best Hits

Swiss-Prot: 100% identical to CCA_PSEFS: Multifunctional CCA protein (cca) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 100% identity to pfs:PFLU5585)

MetaCyc: 57% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Ether lipid metabolism
• Fructose and mannose metabolism
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Riboflavin metabolism
• Sphingolipid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

See C3K334 at UniProt or InterPro

Protein Sequence (409 amino acids)

>PFLU_RS27500 multifunctional CCA addition/repair protein (Pseudomonas fluorescens SBW25-INTG)
MKIYKVGGAVRDRLLGIKVTDIDRVVVGATTEEMLAKGFKPVGADFPVFLDPKNGDEYAL
ARTERKSGRGYGGFVFHASPEVTLEEDLIRRDLTINAMAEDDDGNLTDPYHGLRDLEARI
LRHVSPAFAEDPLRVLRVARFAARYAHLGFTVAPETLELMRQLSESGELEALTPERSWKE
ISRALMEDQPQVFIQVLRDCAALKTLMPEVDALFGVPQPEAHHPEIDTGVHTLSVLEQAA
LHKQPLTVRWACLLHDLGKGTTPVDKLPQHIAHEHRGLKLIKAVNERFKVPRDCQELALL
VGQYHTHGHRALELKASTLLELLQSFDVYRRPQRFEEFAIACEMDARGRKGLEQRIYPQA
DYLRGAAKAAREVAVAPLLEKGYKGPELGEALKRERLKALKAYKEQPIL