Protein Info for PFLU_RS27455 in Pseudomonas fluorescens SBW25-INTG

Annotation: molecular chaperone SurA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF13624: SurA_N_3" amino acids 31 to 149 (119 residues), 54.1 bits, see alignment E=5.3e-18 PF13623: SurA_N_2" amino acids 32 to 113 (82 residues), 35.2 bits, see alignment E=3.4e-12 PF09312: SurA_N" amino acids 33 to 150 (118 residues), 148.7 bits, see alignment E=2.5e-47 PF13145: Rotamase_2" amino acids 160 to 286 (127 residues), 36.3 bits, see alignment E=2.8e-12 amino acids 293 to 391 (99 residues), 33.2 bits, see alignment E=2.5e-11 PF13616: Rotamase_3" amino acids 175 to 282 (108 residues), 51.1 bits, see alignment E=5.8e-17 amino acids 281 to 389 (109 residues), 100.3 bits, see alignment E=3.2e-32 PF00639: Rotamase" amino acids 187 to 278 (92 residues), 64.1 bits, see alignment E=6.1e-21 amino acids 296 to 386 (91 residues), 90.8 bits, see alignment E=2.7e-29

Best Hits

Swiss-Prot: 85% identical to SURA_PSEF5: Chaperone SurA (surA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03771, peptidyl-prolyl cis-trans isomerase SurA [EC: 5.2.1.8] (inferred from 100% identity to pfs:PFLU5576)

MetaCyc: 42% identical to chaperone SurA (Escherichia coli K-12 substr. MG1655)
Peptidylprolyl isomerase. [EC: 5.2.1.8]

Predicted SEED Role

"Survival protein SurA precursor (Peptidyl-prolyl cis-trans isomerase SurA) (EC 5.2.1.8)" in subsystem Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K325 at UniProt or InterPro

Protein Sequence (446 amino acids)

>PFLU_RS27455 molecular chaperone SurA (Pseudomonas fluorescens SBW25-INTG)
MNVKTKLSDCLRPLVLGALLLGTASAHAAVQQLDKVVAIVDNDVIMQSQLDQRVKEVQQT
IAKRGGGVPPTSVLDQQVLERLIVENLQLQIGDRSGIRISDEELNQAVGTIAQRNNMSID
QFRAALAHDGLSYEDARDQIRREMIISRVRQRRVAERVQVSEQEVKNFLASDLGKMQLSE
ELHLANILIPTPDSANSEQLNAAAAKTQAIYDRLKAGADFAQMAIAQSGSDNALEGGDMG
WRKAAQLPPPFDRELSAMEVGGITQPARTPGGFIILKLLEKRGGETSLKDEVHVRHILVK
PSEIRTEAQTKELAQKIYDRIESGEDFATLAKSFSEDPGSALNGGDLNWIDPKALVPEFQ
QVMADTPQGVLSKPFKTQYGWHVLEVLGRRATDNTNQAREQQALNVLRNRKYDEELQTWL
RQIRDEAYVENKLPGAPQTGTDQAAQ