Protein Info for PFLU_RS26850 in Pseudomonas fluorescens SBW25-INTG

Annotation: TonB-dependent receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF07715: Plug" amino acids 44 to 149 (106 residues), 101.6 bits, see alignment E=3.5e-33 PF00593: TonB_dep_Rec_b-barrel" amino acids 183 to 591 (409 residues), 151.8 bits, see alignment E=5.7e-48

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 100% identity to pfs:PFLU5463)

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2R2 at UniProt or InterPro

Protein Sequence (631 amino acids)

>PFLU_RS26850 TonB-dependent receptor (Pseudomonas fluorescens SBW25-INTG)
MNRLRLALPLLLLCAPDLLADTRDDALKLPNVVISANRQVQARNDSSAANTVFTRDDIDR
LQPTRLTDLLSRVPGVQVAPSGGRGSLPGIYMRGTKSAQSLVLVDGQRIANTTSGDSGLQ
YLNVDQIERVEVLRGSRSVIYGSDAIGGVIQVFTRRNAEPGVQPRLKLGFGSHQTWERSL
GLSGGDEQTRFNLGASLDDTAGINSTHTSFPSDGDHDAYRNQSLSLNLSHSFSDGLEAGF
NLLDNRGKSEYDNSFGRYDLATGQTVGQKPYTDYAVSSASGYVDATLNEHWQSRLELGHS
ENRDTKRDTLSDDFSVFNTYRDSVNWQNDLTLNDQNSLILGGDWYEDRFHGSTTFTQNSR
WNRAAFVQHRFHGEWFSTELGLRRDQNQQFGGQNSWSGTVTVPVNPDNDLLLTYSEGFRA
PTFNDLYYPDTQYSNPDLQPETSKSYELQWRSQLSDSTRLEASLYRTDLSDAIILDRSGK
PQNVASARINGFEAALKQELFGWQGNLGLSLIDPRDRDSGHTLARRARQTLNLDLDRQFD
TLGLGASWQAISSSYDDEDNTHRLGGYALLGLRSSWALNRQVALSLKVDNLLDRSYARAR
YSYNDPGFLNNQDYREEGRVWMFGVTWTPES