Protein Info for PFLU_RS26820 in Pseudomonas fluorescens SBW25-INTG

Annotation: NAD(P)H-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 214 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 71 to 92 (22 residues), see Phobius details PF02525: Flavodoxin_2" amino acids 4 to 175 (172 residues), 133.3 bits, see alignment E=9.7e-43 PF03358: FMN_red" amino acids 5 to 108 (104 residues), 52.2 bits, see alignment E=5.4e-18

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5457)

Predicted SEED Role

"Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)" in subsystem Bacterial hemoglobins or Flavohaemoglobin or Glutaredoxins (EC 1.14.12.17)

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.17

Use Curated BLAST to search for 1.14.12.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2Q6 at UniProt or InterPro

Protein Sequence (214 amino acids)

>PFLU_RS26820 NAD(P)H-dependent oxidoreductase (Pseudomonas fluorescens SBW25-INTG)
MAHRTLLILGHPSSTSFCSALADTYTQSAKNAGHEVRALRLGELVFDPVLHNGYSDIQAL
EPDLLSAQSDILWATHLVFVFPIWWGGIPALMKGFLDRIFLPGFAFKYRAGKAFPDKLLQ
GRTAHLLVTLDTPPWYYRWVYRMPGIHQMRATTLAFCGIKPIKTLMFGPILGSTPAHRAK
WLKQAGGLFEKGSFHVHRQSRAVGGHHHQGDSSL