Protein Info for PFLU_RS26735 in Pseudomonas fluorescens SBW25-INTG

Annotation: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 288 to 308 (21 residues), see Phobius details TIGR01081: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase" amino acids 2 to 449 (448 residues), 721.1 bits, see alignment E=3e-221 PF01225: Mur_ligase" amino acids 2 to 100 (99 residues), 81.4 bits, see alignment E=7.6e-27 PF08245: Mur_ligase_M" amino acids 108 to 291 (184 residues), 72.1 bits, see alignment E=9.2e-24 PF02875: Mur_ligase_C" amino acids 311 to 359 (49 residues), 39.4 bits, see alignment 9.1e-14

Best Hits

Swiss-Prot: 59% identical to MPL_ECOLI: UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase (mpl) from Escherichia coli (strain K12)

KEGG orthology group: K02558, UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase [EC: 6.3.2.-] (inferred from 100% identity to pfs:PFLU5439)

MetaCyc: 82% identical to UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase (Pseudomonas aeruginosa PAO1)
RXN0-2361 [EC: 6.3.2.45]

Predicted SEED Role

"UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (EC 6.3.2.-)" (EC 6.3.2.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.-

Use Curated BLAST to search for 6.3.2.- or 6.3.2.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2P1 at UniProt or InterPro

Protein Sequence (449 amino acids)

>PFLU_RS26735 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (Pseudomonas fluorescens SBW25-INTG)
MHIHILGICGTFMGSMAVLAKELGHHVTGSDANVYPPMSTQLEAQGIALTQGYDPAQFNP
VPDLVVIGNAMSRGNPAVEYVLNKGLPYVSGPQWLADHVLQGRWVLAVAGTHGKTTTSSM
LAWVLEHAGMSPGFLIGGVPQNFSVSARLGDTPFFVIEADEYDSAFFDKRSKFVHYRPRT
AILNNLEFDHADIFPDLPAIERQFHHLVRTIPSEGLVIHPTTEPALQRVIEMGCWTPVQT
TGAGGQWQVKLLSEDGSKFEVLFDGEAQGIVDWDMTGQHNVANALVTLAAARHVGVVPSM
GIAALSAFKSVKRRMEKVADVNGITIYDDFAHHPTAIATTLDGLRKRVGDAPIIAIIEPR
SNSMKLGAHRDGLPESVNDADEAVWYAPANLGWDLPGIAALCTVPSTVCDSIEGIIEHVR
QQAKPGTHVVVMSNGGFGGLHGKLAEALK