Protein Info for PFLU_RS26635 in Pseudomonas fluorescens SBW25-INTG

Annotation: D-alanyl-D-alanine carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00768: Peptidase_S11" amino acids 28 to 255 (228 residues), 295.7 bits, see alignment E=3.9e-92 PF13354: Beta-lactamase2" amino acids 39 to 207 (169 residues), 53.8 bits, see alignment E=2.8e-18 PF07943: PBP5_C" amino acids 275 to 365 (91 residues), 95.5 bits, see alignment E=2.6e-31

Best Hits

Swiss-Prot: 46% identical to DACC_ECOLI: D-alanyl-D-alanine carboxypeptidase DacC (dacC) from Escherichia coli (strain K12)

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 100% identity to pfs:PFLU5419)

MetaCyc: 46% identical to D-alanyl-D-alanine carboxypeptidase DacC (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4 or 3.4.17.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2M1 at UniProt or InterPro

Protein Sequence (385 amino acids)

>PFLU_RS26635 D-alanyl-D-alanine carboxypeptidase (Pseudomonas fluorescens SBW25-INTG)
MNITTLAKRTCLLISLIITPAAWAVEMVPASPQLAAKSWVLMDAASGNVLVESNGDQRLP
PASLTKLMTAYIATLEIRRGQIGENDPVTVSENAWRTGGSRMFIKVGSQVTVSDLLHGII
IQSGNDASVALAEHIAGSEDAFADMMNKTVADLGMTNSHFMNPTGLPNPEHYSSAHDMAI
LARAIIRVDPVHYAIYSQKEFFWNNIKQPNRNLLLWRDKTVDGLKTGHTDEAGYCMVSSA
VRDGQRLIAVVFGTNSEQARAAETQKLLTYGFRFFETQTFYQKGAELATAPVWKGATSQV
KAGLAEDLTLTMPKGQLKKLAASMTMNPQLVAPIAKGDVIGKVEVKLDDKVVHSADLIAL
DAVDEGGIFRRVWDSIRLFFYSLFN