Protein Info for PFLU_RS25950 in Pseudomonas fluorescens SBW25-INTG

Annotation: FAD-binding oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 936 PF01565: FAD_binding_4" amino acids 41 to 177 (137 residues), 114.2 bits, see alignment E=1.4e-36 PF02913: FAD-oxidase_C" amino acids 267 to 513 (247 residues), 209 bits, see alignment E=3.5e-65 PF13183: Fer4_8" amino acids 537 to 608 (72 residues), 40.4 bits, see alignment 1.3e-13 PF13534: Fer4_17" amino acids 539 to 609 (71 residues), 33.5 bits, see alignment 1.8e-11 PF12838: Fer4_7" amino acids 539 to 608 (70 residues), 35.2 bits, see alignment 5e-12 PF02754: CCG" amino acids 705 to 776 (72 residues), 18.7 bits, see alignment 5.4e-07

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5279)

Predicted SEED Role

"Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K285 at UniProt or InterPro

Protein Sequence (936 amino acids)

>PFLU_RS25950 FAD-binding oxidoreductase (Pseudomonas fluorescens SBW25-INTG)
MSLPAAFLSDVAHLIPKDRRFDDALSTLAFGTDASFYRLIPQLVIRVESEDEVVTLLQLA
QRDRVPVTFRAAGTSLSGQAISDSVLIVLGDNWSGREIRGQGTQIRLQPGVIGAQANAWL
APFGRKIGPDPASINACKIGGIVANNASGMCCGTAQNTYHTLAGIRLVLADGSRVDTEDA
ASVKAFREQHGPLLERLATLGRETRANAELAAKIRHKYRLKNTTGLSLNALVDFDEPLDI
LSHLLVGSEGTLGFISAVTYDTVIDHPNKASALIVFPDVETCCNAVSVLKTQPVSAVELL
DRRSLRSVQNKPGMPTFVQQLSETACALLIESRAASTSLLHEQLALIMASLAQFPVEKQV
DFTEDPVENARLWAIRKDTFPAVGAVRKTGTTVIIEDVTFPVEQLAIGVNRLIELFDKHH
YDEAILFGHALEGNLHFVFTQGFNSAEEVTRYQAFMDDVAQLVAVEFGGSLKAEHGTGRN
MAPFVELEWGSDAYQLMWQLKRLLDPNGILNPDVVLSEDPHIHLKHLKPLPAADELVDKC
IECGFCEPVCPSKGLTLSPRQRIVIWRDIQAKKRAGVDTTELEAAYHYQGIDTCAATGLC
AQRCPVGINTGDLVKKLRSRDADRTKTAEWLATHFATALQGARFTLHVANGARMLLGAPR
LAKLSASVTKLSKGQIPQWTPAMPQPEKAIRFSPAVTDERPRVVYLAACVSRAMGPAAGD
KEQMSLYDKTRNLLEKAGYQVVFPDNQDSLCCGQPFASKGYAEQAEHKRQELIGALLHAS
RGGLDPIYCDTSPCTLRLVQDLSETRLDLYDPVRFIRTHLMDRLDFTPQEAPIAVHVTCS
TQHLGESQALIDLARRCSNTVVIPEGIHCCGFAGDKGFTTPELNAHSLRTLKGAVQYCSE
GISTSRTCEIGLSQHGGIDYHGLVYLVDRVTQARAH