Protein Info for PFLU_RS25765 in Pseudomonas fluorescens SBW25-INTG
Annotation: acetate--CoA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to ACSA2_PSEPK: Acetyl-coenzyme A synthetase 2 (acsA2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to pfs:PFLU5244)MetaCyc: 60% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]
Predicted SEED Role
"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)
MetaCyc Pathways
- superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
- 2-methylcitrate cycle I (5/5 steps found)
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- superpathway of acetate utilization and formation (3/3 steps found)
- β-alanine biosynthesis II (5/6 steps found)
- 2-methylcitrate cycle II (5/6 steps found)
- acetate and ATP formation from acetyl-CoA III (1/1 steps found)
- acetate conversion to acetyl-CoA (1/1 steps found)
- L-isoleucine biosynthesis V (2/3 steps found)
- ethanol degradation III (2/3 steps found)
- L-isoleucine biosynthesis IV (4/6 steps found)
- chitin deacetylation (2/4 steps found)
- acrylate degradation II (1/3 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (5/9 steps found)
- cis-geranyl-CoA degradation (4/9 steps found)
- adlupulone and adhumulone biosynthesis (1/6 steps found)
- colupulone and cohumulone biosynthesis (1/6 steps found)
- lupulone and humulone biosynthesis (1/6 steps found)
- superpathway of bitter acids biosynthesis (3/18 steps found)
KEGG Metabolic Maps
- Glycolysis / Gluconeogenesis
- Propanoate metabolism
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.1
Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K250 at UniProt or InterPro
Protein Sequence (645 amino acids)
>PFLU_RS25765 acetate--CoA ligase (Pseudomonas fluorescens SBW25-INTG) MFDISTFPTADAVRRAAQLSQEDYQRLYRQSIEQPDTFWAEQAKAFLDWITPWHTVHRSD INTGAAQWFAGGQLNVSYNCIDRHLGQRADQPAFIWEGDDPAKSSTITYRQLHQNVSRLA NVLKSRGVKKGDRVCIYMPMIPEAAYAMLACTRIGAVHSVVFGGFSPDALRDRILDADCR TVITADEGVRGGKPVALKQNVDKALASCPNVSTVLVVERTGADLIWVKDRDIKYRQALDA ASDDCPPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTFKYVLDYRDGEVFWC TADVGWVTGHSYIVYGPLANGATSLMFEGVPSYPDSSRFWQVIDKHKVNIFYTAPTALRA LMREGHGPLENTSRASLRLLGSVGEPINPEAWDWYFNAVGEQRCPIVDTWWQTETGGIML SPLVSAQRIKPGCATQPMFGVQPVLLDEHGKAFSGAGSGVLAIKASWPGQIRSVYGDPQR MIDTYFKPYPGYYFTGDGARRDEDGDYWITGRIDDVINVSGHRIGTAEVESALVLHDQVA EAAVVGYPHDVKGQGIYAFVTPMNGVEPSDALKKHLLELVSKEIGSFAKPELIQWAPALP KTRSGKIMRRILRKIACNELDSLGDTSTLADPSVVEGLIDKRLNR