Protein Info for PFLU_RS25715 in Pseudomonas fluorescens SBW25-INTG

Annotation: RNA polymerase-binding protein DksA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 146 TIGR02420: RNA polymerase-binding protein DksA" amino acids 27 to 135 (109 residues), 169.6 bits, see alignment E=1.2e-54 PF21157: DksA_CC" amino acids 32 to 101 (70 residues), 112.8 bits, see alignment E=7.3e-37 PF01258: zf-dskA_traR" amino acids 105 to 138 (34 residues), 42.9 bits, see alignment 3.7e-15

Best Hits

Swiss-Prot: 71% identical to DKSA_SALTI: RNA polymerase-binding transcription factor DksA (dksA) from Salmonella typhi

KEGG orthology group: K06204, DnaK suppressor protein (inferred from 100% identity to pfs:PFLU5233)

Predicted SEED Role

"C4-type zinc finger protein, DksA/TraR family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K240 at UniProt or InterPro

Protein Sequence (146 amino acids)

>PFLU_RS25715 RNA polymerase-binding protein DksA (Pseudomonas fluorescens SBW25-INTG)
MSTQAKQQQQSLSGFTPYVEAKGEEYMGKPMRQHFTNILKKWKQDLMQEVDRTVDHMKDE
AANFPDPADRASQEEEFALELRARDRERKLIKKIDKTLQLIEDEEYGWCESCGVEIGIRR
LEARPTADLCVDCKTLAEIKEKQVGK