Protein Info for PFLU_RS24900 in Pseudomonas fluorescens SBW25-INTG

Annotation: tRNA guanosine(34) transglycosylase Tgt

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 TIGR00430: tRNA-guanine transglycosylase" amino acids 6 to 368 (363 residues), 548.5 bits, see alignment E=7.1e-169 TIGR00449: tRNA-guanine family transglycosylase" amino acids 6 to 367 (362 residues), 521.7 bits, see alignment E=9e-161 PF01702: TGT" amino acids 14 to 366 (353 residues), 545.1 bits, see alignment E=3.9e-168

Best Hits

Swiss-Prot: 95% identical to TGT_PSESM: Queuine tRNA-ribosyltransferase (tgt) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 100% identity to pfs:PFLU5077)

MetaCyc: 69% identical to tRNA-guanine transglycosylase (Escherichia coli K-12 substr. MG1655)
tRNA-guanine transglycosylase. [EC: 2.4.2.29]

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1N4 at UniProt or InterPro

Protein Sequence (374 amino acids)

>PFLU_RS24900 tRNA guanosine(34) transglycosylase Tgt (Pseudomonas fluorescens SBW25-INTG)
MSRMSFELLATDGKARRGRLTFPRGTVETPAFMPVGTYGTVKGMLPRDIVATGAEIILGN
TFHLWLRPGTEVIKKHGDLHDFMKWQGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDG
SKVFMGPEESMQVQRDLGSDIVMIFDECTPYPADEDVARISMELSLRWAQRSKNAHGDNT
AALFGIVQGGMHESLRKRSLEGLDKIGFDGLAIGGLSVGEPKHEMIKVLDYLPGLMPADK
PRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFIDTGVLKIRNAFHRHDDSPLD
PTCDCYTCQNFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQVLMAGLREAIQQGTLAAFVD
AFYAKRGLPVPPLD