Protein Info for PFLU_RS24000 in Pseudomonas fluorescens SBW25-INTG

Annotation: aminomethyl-transferring glycine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 946 transmembrane" amino acids 744 to 759 (16 residues), see Phobius details PF02347: GDC-P" amino acids 14 to 438 (425 residues), 609.5 bits, see alignment E=5.4e-187 amino acids 452 to 729 (278 residues), 41.9 bits, see alignment E=9.5e-15 TIGR00461: glycine dehydrogenase" amino acids 14 to 939 (926 residues), 1498.3 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 768 to 889 (122 residues), 207.8 bits, see alignment E=5.3e-66

Best Hits

Swiss-Prot: 94% identical to GCSP1_PSEPF: Glycine dehydrogenase (decarboxylating) 1 (gcvP1) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 62% identity to ajs:Ajs_1936)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JYR1 at UniProt or InterPro

Protein Sequence (946 amino acids)

>PFLU_RS24000 aminomethyl-transferring glycine dehydrogenase (Pseudomonas fluorescens SBW25-INTG)
MTVNLTTANEFIARHIGPRQEDEQHMLASLGFDSLEALSASVIPESIKGTSVLGLEDGLS
EAEALAKIKAIAGQNQLFKTYIGQGYYNCHTPSPILRNLLENPAWYTAYTPYQPEISQGR
LEALLNFQTLISDLTGLPIANASLLDEATAAAEAMTFCKRLSKNKGSNAFFASIHSHPQT
LDVLRTRAEPLGIDVVVGDERELTDVSAFFGALLQYPASNGDVFDYRALTERFHASNALV
AVAADLLALTLLTPPGEFGADVAIGSAQRFGVPLGFGGPHAAYFSTKDAFKRDMPGRLVG
VSVDRFGKPALRLAMQTREQHIRREKATSNICTAQVLLANIASMYAVYHGPKGLTQIAQR
IHQLTAILAKGLTALGQKVEQEHFFDTLTLNTGANTATLHDKARAQRINLRVVDAERLGV
SVDETTTQADIETLWAIFADGKALPDFAANVDSTLPAALLRQSPILSHPVFNRYHSETEL
MRYLRKLADKDLALDRTMIPLGSCTMKLNAASEMIPVTWAEFGALHPFAPAEQSAGYLQL
TSDLEAMLCAATGYDAISLQPNAGSQGEYAGLLAIRAYHQSRGEERRDICLIPSSAHGTN
PATANMAGMRVVVTACDARGNVDIEDLRAKAIEHRDHLAALMITYPSTHGVFEEGIREIC
GIIHDNGGQVYIDGANMNAMVGLCAPGKFGGDVSHLNLHKTFCIPHGGGGPGVGPIGVKS
HLTPFLPGHAAMERKEGAVCAAPFGSASILPITWMYISMMGGAGLKRASQLAILNANYIS
RRLEEHYPVLYTGSNGLVAHECILDLRPLKDSSGISVDDVAKRLIDFGFHAPTMSFPVAG
TLMIEPTESESKEELDRFCDAMIAIREEIRAVENGTLDKDDNPLKNAPHTAAELVGEWSH
PYSREQAVYPVASLIEGKYWPPVGRVDNVFGDRNLVCACPSIESYA