Protein Info for PFLU_RS23165 in Pseudomonas fluorescens SBW25-INTG

Annotation: flagellar hook protein FlgE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR03506: flagellar hook-basal body protein" amino acids 1 to 416 (416 residues), 302.4 bits, see alignment E=3.3e-94 PF00460: Flg_bb_rod" amino acids 4 to 33 (30 residues), 32.9 bits, see alignment (E = 7.4e-12) PF07559: FlaE" amino acids 180 to 315 (136 residues), 78.1 bits, see alignment E=1.4e-25 PF06429: Flg_bbr_C" amino acids 355 to 433 (79 residues), 60.9 bits, see alignment E=1.5e-20

Best Hits

KEGG orthology group: K02390, flagellar hook protein FlgE (inferred from 100% identity to pfs:PFLU4728)

Predicted SEED Role

"Flagellar hook protein FlgE" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0Q7 at UniProt or InterPro

Protein Sequence (434 amino acids)

>PFLU_RS23165 flagellar hook protein FlgE (Pseudomonas fluorescens SBW25-INTG)
MSFNIGLSGLYAANKQLDVTGNNIANVATTGFKSSRAEFSDIYAASKLGTGQNSIGNGVN
LAAVSQQFTQGDVNNSGGVLDMAIQGGGFFVQKGSDGSLEYTRSGAFRADKDGYITNNTG
TSRLQGYAADADGNIVKGGLTDLQLNLSYLPPKASTKVDSTSNLNSSSPVIDQATKLFDP
TDTSTFTTQNSTTLYDAQGNSHEMVQYMVKSNPNEWKAYTLIDGRNLDGTKPTTTGATAP
VPATYTFDGAGKLLGVTGTGVVGTTLTLTGWVPGTVTNGNWAPNNATAPNIAVNLGNVTQ
YNSASYRNPPTTDGYATGQITGLKIDGSGVLFATFSNQQSKAIGQISLASFNNEQGLQPS
GGTTWRETFASGQPGYDTPQAGTLGSIVANSLENSNVNLTNELVDLIKAQSNYQANAKTI
STQSTIMQTIIQMT