Protein Info for PFLU_RS22525 in Pseudomonas fluorescens SBW25-INTG

Annotation: benzoate/H(+) symporter BenE family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 transmembrane" amino acids 17 to 40 (24 residues), see Phobius details amino acids 52 to 72 (21 residues), see Phobius details amino acids 79 to 97 (19 residues), see Phobius details amino acids 103 to 122 (20 residues), see Phobius details amino acids 129 to 146 (18 residues), see Phobius details amino acids 152 to 170 (19 residues), see Phobius details amino acids 177 to 199 (23 residues), see Phobius details amino acids 215 to 234 (20 residues), see Phobius details amino acids 261 to 265 (5 residues), see Phobius details amino acids 299 to 322 (24 residues), see Phobius details amino acids 328 to 349 (22 residues), see Phobius details amino acids 360 to 391 (32 residues), see Phobius details PF03594: BenE" amino acids 14 to 388 (375 residues), 524.3 bits, see alignment E=8.6e-162 TIGR00843: benzoate transporter" amino acids 16 to 391 (376 residues), 437.8 bits, see alignment E=1.8e-135

Best Hits

Swiss-Prot: 44% identical to BENE_ACIAD: Benzoate membrane transport protein (benE) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K05782, benzoate membrane transport protein (inferred from 100% identity to pfs:PFLU4597)

Predicted SEED Role

"Benzoate transport protein" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JYN1 at UniProt or InterPro

Protein Sequence (396 amino acids)

>PFLU_RS22525 benzoate/H(+) symporter BenE family transporter (Pseudomonas fluorescens SBW25-INTG)
MTDATQAPLRPLADTSASAVVAGFIAMMTGYTSSLVLMFQAGQAAGLTTAQISSWIWAIS
IGMAVCSIGLSLRYRTPITIAWSTPGAALLITSLGGVSYGEAIGAYITCAVLVTICGLTG
SFEKLVKRIPASLAAALLAGILFKIGSEIFVAAQHRTGLVLGMFFTYLVIKRLSPRYAVL
AALLIGTALSGLMGLLDFSAFHLEVATPVWTTPHFSLAATISIGIPLFVVAMTSQNMPGV
AVLRADGYNVPASPLITATGLASLVLAPFGSHGINLAAISAAICTGPHAHEDRNKRYTAA
VWCGVFYGIAGVFGATLAALFAALPKELVLSIAALALFGSIINGLSIAMNEPKEREAALI
TFMVTASGLTLFSIGSAFWGIVAGVLTLLILNGRKA