Protein Info for PFLU_RS21745 in Pseudomonas fluorescens SBW25-INTG

Annotation: fused response regulator/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 572 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00072: Response_reg" amino acids 13 to 124 (112 residues), 81.3 bits, see alignment E=8.7e-27 PF07228: SpoIIE" amino acids 199 to 386 (188 residues), 135.1 bits, see alignment E=4.6e-43 PF13581: HATPase_c_2" amino acids 496 to 564 (69 residues), 31.8 bits, see alignment E=2e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4432)

Predicted SEED Role

"Serine phosphatase RsbU, regulator of sigma subunit / Serine-protein kinase RsbW (EC 2.7.11.1)" in subsystem SigmaB stress responce regulation (EC 2.7.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0T9 at UniProt or InterPro

Protein Sequence (572 amino acids)

>PFLU_RS21745 fused response regulator/phosphatase (Pseudomonas fluorescens SBW25-INTG)
MSVLAPVAQALSVLIAEDSAADLLLLSTIIRRQGHQVLTATNGAEAVEVFTRERPQLVLM
DALMPVMDGFEAARQIKQLAGEALVPIIFLTSLRQSEALAQCLDAGGDDFLPKPYNPLIL
AAKINAMDRLRRLQATVLQQRDLIAKHHDYLLHEQRVAKAVFDKVAHSGCINAAPNIRYL
QSPYALFNGDLLLAAYTPSGDMHVLLGDFTGHGLPAAVGAMPLAEVFYGMTAKGYGLAQT
LREMNAKLKRILPVDMFCCATLLCLSTQRRVVEVWNGGMPDGYVHEVATGKRTPLASRHL
PLGVLSAETFDDRTEVWPMALGDRVFLLSDGVLDTADANDQLFGAERLQQVFAANREPDR
LFEAIEQALAAFRGEARDDVSMVEITLQAGPPLRASGPLYADSGQSCPLDWSVSFEFRAE
TLKRYNPLPYLLQLLLEIHGLREQSGALYSVMAELYSNALEHGVLGLDSRLKRDADGFAE
YYRQRNDRLAQLDSGHIRVHVQVVPTETGGTMTLRVEDSGLGFDVEQVLARPLDIDRLSG
RGLSLVRQLSSAVRWSDGGRSVCVEFSWEALA