Protein Info for PFLU_RS21445 in Pseudomonas fluorescens SBW25-INTG

Annotation: RluA family pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 PF00849: PseudoU_synth_2" amino acids 15 to 161 (147 residues), 110.1 bits, see alignment E=6.1e-36

Best Hits

Swiss-Prot: 54% identical to RLUA_YERPE: Ribosomal large subunit pseudouridine synthase A (rluA) from Yersinia pestis

KEGG orthology group: K06177, ribosomal large subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to pfs:PFLU4372)

MetaCyc: 53% identical to 23S rRNA pseudouridine746 and tRNA pseudouridine32 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11843 [EC: 5.4.99.29]; RXN-11842 [EC: 5.4.99.29, 5.4.99.28]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.28 or 5.4.99.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0D6 at UniProt or InterPro

Protein Sequence (211 amino acids)

>PFLU_RS21445 RluA family pseudouridine synthase (Pseudomonas fluorescens SBW25-INTG)
MPLSNIHILYQDAAVLVVNKPTLLLSVPGRADDNKDCLITRLQENGYPEARIVHRLDWET
SGIILLARDADTHRELSRQFHDRETEKAYTALAWGQPELDSGSIDLPLRYDPPTKPRHVV
DHEFGKHALTFWKVLERCGDWCRVELTPITGRSHQLRVHMLSIGHPLLGDGLYAHEQALA
AWPRLCLHASMLSFTHPQSGERLRFECPAPF