Protein Info for PFLU_RS21425 in Pseudomonas fluorescens SBW25-INTG

Annotation: lipid A biosynthesis lauroyl acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02207: lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase
  • amino acids 4 to 306 (303 residues), 428 bits, E=1.1e-132
• PF03279: Lip_A_acyltrans
  • amino acids 5 to 296 (292 residues), 277.3 bits, E=7.5e-87

Best Hits

Swiss-Prot: 45% identical to LPXL_ECO57: Lipid A biosynthesis lauroyltransferase (lpxL) from Escherichia coli O157:H7

KEGG orthology group: K02517, lipid A biosynthesis lauroyl acyltransferase [EC: 2.3.1.-] (inferred from 100% identity to pfs:PFLU4368)

MetaCyc: 45% identical to lauroyl acyltransferase (Escherichia coli K-12 substr. MG1655)
LAUROYLACYLTRAN-RXN [EC: 2.3.1.241]

Predicted SEED Role

"Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-)" in subsystem KDO2-Lipid A biosynthesis (EC 2.3.1.-)

MetaCyc Pathways

• superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
• (Kdo)₂-lipid A biosynthesis (E. coli) (1/2 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (17/25 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.- or 2.3.1.241

Sequence Analysis Tools

See C3K0D2 at UniProt or InterPro

Protein Sequence (310 amino acids)

>PFLU_RS21425 lipid A biosynthesis lauroyl acyltransferase (Pseudomonas fluorescens SBW25-INTG)
MDRPRFRAVFFHPRFWLLWLGLGLLWLVTQLPYRALLTIGRLLGAGMYRVAGERRRIAAR
NLELCFPEKSAKERKRLLKENFASTGIAFFEMAMSWWWSRQRLARLAHVEGLEHLKQAQL
DGKGVILMALHFTTLEIGAALLGQKHTIDGMYREHGNPLFDFVQRRGRERHNLDSLAVER
EDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGKALVVPFT
QERLADGSGYRLVIHPPLTDFPGESDEVDCLRINQWVEASVRECPEQYLWTHRRFKSRPP
GEAKLYEKRR