Protein Info for PFLU_RS21205 in Pseudomonas fluorescens SBW25-INTG

Annotation: N-acetylglutaminylglutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 577 TIGR03103: GNAT-family acetyltransferase TIGR03103" amino acids 37 to 577 (541 residues), 887.5 bits, see alignment E=1.6e-271 PF00583: Acetyltransf_1" amino acids 152 to 245 (94 residues), 53.1 bits, see alignment E=1.7e-17 PF13508: Acetyltransf_7" amino acids 157 to 246 (90 residues), 39.1 bits, see alignment E=3.5e-13 PF08445: FR47" amino acids 188 to 249 (62 residues), 27.6 bits, see alignment E=1e-09 PF13673: Acetyltransf_10" amino acids 190 to 250 (61 residues), 24.6 bits, see alignment E=1e-08 PF08443: RimK" amino acids 329 to 433 (105 residues), 38 bits, see alignment E=6.3e-13 PF02955: GSH-S_ATP" amino acids 354 to 435 (82 residues), 28.4 bits, see alignment E=4.7e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4321)

Predicted SEED Role

"Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30)" in subsystem Cyanophycin Metabolism (EC 6.3.2.29, EC 6.3.2.30)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.29 or 6.3.2.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K069 at UniProt or InterPro

Protein Sequence (577 amino acids)

>PFLU_RS21205 N-acetylglutaminylglutamine synthetase (Pseudomonas fluorescens SBW25-INTG)
MKPLAAAYSQRLLKGQAPTYERLQARLAEDGSPLAAEPIAVHCGWGRLLIGHTFPDPATL
AQELLNEQPGERDIALYVAAPQQILGIDPQQLFLDPSDTLRLWFTDYRPATRVFRGFRIR
RVQTEADWLAVNQLYQGRGMLPVDAERLTPRHAGGPVYWLAEDEDSGAVIGSVMGLNHQK
AFHDPENGCSLWCLAVDPQCTRPGVGEVLVRHLVEHFMSRGLSYLDLSVLHDNRQAKNLY
AKLGFRALTTFAIKRKNGINQPLFLGPGPQAGFNPYARIIVEEAHRRGIDVQVDDADAGL
FTLSHGGRRVRCRESLSDLTSAISMTLCQDKSLTHKVLKAAGLKLPSQQLAGSADDNLEF
LDEHQRVVVKPLDGEQGQGVAVDLQSIEEVQQAIEAARQFDSRVLLESFHEGLDLRILVI
GFEVVAAAIRRPAEVTGDGQHSIGALIEAQSRRRQAATDGESKIPVDAETERTLHAAGYD
YSSILPRGKTLAVRRTANLHTGGCLEDVTAILHPTLVDAAVRAARALDIPMVGLDLMVPA
ADQPEYVFIEANERAGLANHEPQPTAEKFVDLLFPHS