Protein Info for PFLU_RS19475 in Pseudomonas fluorescens SBW25-INTG

Annotation: Zn-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 18 to 414 (397 residues), 433.5 bits, see alignment E=3.8e-134 PF04389: Peptidase_M28" amino acids 72 to 153 (82 residues), 26.4 bits, see alignment E=8e-10 PF01546: Peptidase_M20" amino acids 89 to 413 (325 residues), 104.5 bits, see alignment E=1.1e-33 PF07687: M20_dimer" amino acids 221 to 319 (99 residues), 27.1 bits, see alignment E=5.2e-10

Best Hits

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 100% identity to pfs:PFLU3987)

Predicted SEED Role

"N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)" in subsystem Hydantoin metabolism (EC 3.5.1.87)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JYY2 at UniProt or InterPro

Protein Sequence (422 amino acids)

>PFLU_RS19475 Zn-dependent hydrolase (Pseudomonas fluorescens SBW25-INTG)
MNSFAQPLKSNAPLINRDRLWQSLMDLARLGATAKGGVCRLALTDLDRQARDLFVQWCEA
AGCSVSVDAIGNIFARRAGRNPALLPVMTGSHIDTQPTGGKFDGCYGVMAGLEVIRTLND
LNIETEAPIEVVVWTNEEGSRFPPCMMGSGVFAGKFDLQDTLDKVDDQGLSVGAELQRIG
YAGSRAALGHPVGAYFEAHIEQGPVLEDQATTIGVVMGCLGQKWFDLTFTGVEAHAGPTP
MHLRKDALVGAAEVVSAVNRIAHQQQPHACGTVGCLSLHPGSRNVIPGQVHMTLDLRHLH
ADTLQAMVDEVRGVIEATATRHGLTFELTPTADFPPLDFAPACVNAVREGAEALGLSHMD
IVSGAGHDAIFVAELGPAGMIFVPCEGGISHNEIENAAPDDLAAGCAVLLRAMVNAAQGE
IA