Protein Info for PFLU_RS19400 in Pseudomonas fluorescens SBW25-INTG

Annotation: IS110 family transposase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 transmembrane" amino acids 187 to 208 (22 residues), see Phobius details amino acids 282 to 297 (16 residues), see Phobius details PF01548: DEDD_Tnp_IS110" amino acids 7 to 147 (141 residues), 77.6 bits, see alignment E=9.2e-26 PF02371: Transposase_20" amino acids 188 to 271 (84 residues), 55.5 bits, see alignment E=6.1e-19

Best Hits

KEGG orthology group: K07486, transposase (inferred from 100% identity to pfs:PFLU1732A)

Predicted SEED Role

"Mobile element protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>PFLU_RS19400 IS110 family transposase (Pseudomonas fluorescens SBW25-INTG)
MFSYPAGIDVSSGSLEIRVDQLDVAMSCSNSQSDFPGLIGWLTLHRVGRVLLEATGGYER
KVMKALQAAGFEVIRINPCRAKSFARAMGQQAKTDPIDARLLAQFAAVIKSPDSRVTSAE
QDDLRALVQQREHFVQQRDDDKRRLKTTSSDVVKPALQSHIDYLQKVLKELESLIRQSAE
RLDRKKVALLCSVKGIGLVTAASLMSYLPELGEVGRHQIAALSGTAPYNDDSGKHKGKRH
ISGGRFAARRSLYMACWSVIRFQPEFAARYKALREKGKCAKVALIACMRVLLVRLNAMIR
DGSEWKEHAA