Protein Info for PFLU_RS19390 in Pseudomonas fluorescens SBW25-INTG

Annotation: ornithine cyclodeaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 62% identical to OCD_PSEPK: Ornithine cyclodeaminase (ocd) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01750, ornithine cyclodeaminase [EC: 4.3.1.12] (inferred from 100% identity to pfs:PFLU3970)

MetaCyc: 58% identical to ornithine cyclodeaminase (Agrobacterium tumefaciens)
Ornithine cyclodeaminase. [EC: 4.3.1.12]

Predicted SEED Role

"Ornithine cyclodeaminase (EC 4.3.1.12)" in subsystem Arginine and Ornithine Degradation (EC 4.3.1.12)

MetaCyc Pathways

L-arginine degradation VII (arginase 3 pathway) (2/2 steps found)
L-ornithine degradation I (L-proline biosynthesis) (1/1 steps found)
L-proline biosynthesis II (from arginine) (1/2 steps found)

KEGG Metabolic Maps

Arginine and proline metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.12

Use Curated BLAST to search for 4.3.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JYW5 at UniProt or InterPro

Protein Sequence (348 amino acids)

>PFLU_RS19390 ornithine cyclodeaminase (Pseudomonas fluorescens SBW25-INTG)
MTRYIDVNDLSYLVAQKGLQTCLTEMAEYIRADYLRWQDFEKCARLANHSPDGVIELMPV
SDASLYAFKYVNGHPKNTLAGMLTVMAFGALGDVDTGKPVLLAEMTLTTAIRTAATSALV
ARYLARENSRSMALIGNGSQSEFQALAFHAMLGITEIRLFDIDAKATAKLADNLKAFPAI
KVILAGSVAEAVKGADIVTTVTADKAYATILTDEMIEPGMHLNAVGGDCPGKTELDRRIV
ERARVIVEYEPQSRIEGEIQQMPHDSPVTELWQVINGQQPGRENAHQITLFDSVGFAIED
YSALRYVLDVAKALDVGSELELVPDLADPKDLFARLAQQPRVQQQKRA