Protein Info for PFLU_RS18960 in Pseudomonas fluorescens SBW25-INTG
Annotation: AMP-binding protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 100% identity to pfs:PFLU3888)Predicted SEED Role
"Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 6.2.1.16)
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
- superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
- palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
- 2-deoxy-D-ribose degradation II (7/8 steps found)
- palmitate biosynthesis III (21/29 steps found)
- sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
- stearate biosynthesis I (animals) (1/6 steps found)
- ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis II
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Butanoate metabolism
- Caprolactam degradation
- Ethylbenzene degradation
- Geraniol degradation
- Limonene and pinene degradation
- Propanoate metabolism
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.-, 6.2.1.16
Use Curated BLAST to search for 6.2.1.- or 6.2.1.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3JYF0 at UniProt or InterPro
Protein Sequence (545 amino acids)
>PFLU_RS18960 AMP-binding protein (Pseudomonas fluorescens SBW25-INTG) MDQSNQSYSRGPQDKALLAMTIGQAFDRTVARHPDGEALVVRHQQLRYTWRQLAEAVDVH ARAFLALGMQAGDRLGIWAPNCAEWCISQIASAKLGVILVNINPAYRSSELDYVLKQSGC QWLVCAGSFKTSDYHAMLQALKPDLRGMISLDPNPPPGFLPWSQLAARGAGIPLEQLHAR QAALHFDQPVNIQYTSGTTGFPKGATLSHHNILNNGYMVGESLGLTAQDRLVIPVPLYHC FGMVMGNLGCITHGTTMIYPNDGFDPLLTLAAVAEERATGLYGVPTMFIAMLDHPRLGDF DLSTLRTGIMAGATCPIEVMRRVISEMHMSEVQIAYGMTETSPVSLQTGADDDLERRVTT VGRTQPQLENKIIDADGNTIARGEIGELCTRGYSVMLGYWNNPDATREAIDDAGWMHTGD LASMDAHGYVCIAGRNKDMIIRGGENVYPRELEEFFFTHPAVADVQVIGIPDERYGEAIV AWIKFHPGHVANELELQTWCKGRIAHFKTPKYFKFVEAFPMTVTGKIQKFRMREITIEEL RTQSQ