Protein Info for PFLU_RS18665 in Pseudomonas fluorescens SBW25-INTG

Annotation: NADH-quinone oxidoreductase subunit L

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 31 to 50 (20 residues), see Phobius details amino acids 62 to 82 (21 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 117 to 134 (18 residues), see Phobius details amino acids 140 to 160 (21 residues), see Phobius details amino acids 172 to 192 (21 residues), see Phobius details amino acids 212 to 230 (19 residues), see Phobius details amino acids 250 to 269 (20 residues), see Phobius details amino acids 281 to 302 (22 residues), see Phobius details amino acids 309 to 329 (21 residues), see Phobius details amino acids 334 to 357 (24 residues), see Phobius details amino acids 376 to 398 (23 residues), see Phobius details amino acids 416 to 436 (21 residues), see Phobius details amino acids 456 to 478 (23 residues), see Phobius details amino acids 498 to 517 (20 residues), see Phobius details amino acids 529 to 547 (19 residues), see Phobius details amino acids 596 to 616 (21 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 2 to 612 (611 residues), 778.1 bits, see alignment E=4.7e-238 PF00662: Proton_antipo_N" amino acids 69 to 119 (51 residues), 68.5 bits, see alignment 3.7e-23 PF00361: Proton_antipo_M" amino acids 136 to 427 (292 residues), 295.3 bits, see alignment E=4.7e-92

Best Hits

Swiss-Prot: 83% identical to NUOL_PSEAE: NADH-quinone oxidoreductase subunit L (nuoL) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 100% identity to pfs:PFLU3828)

MetaCyc: 90% identical to NADH-quinone oxidoreductase subunit L (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JY91 at UniProt or InterPro

Protein Sequence (617 amino acids)

>PFLU_RS18665 NADH-quinone oxidoreductase subunit L (Pseudomonas fluorescens SBW25-INTG)
MNMIFLTFVFPLIGFLLLSFSRGRWSENLSALVGVGSIGLSAIVAAYVIWQFNVAPPEGG
HYTLVLWQWMSVEGFKPNFALYVDGLSITMLGVVVGVGFLIHLFASWYMRGEAGYSRFFS
YTNLFIASMLFLVLGDNLLFLYFGWEGVGLCSYLLIGFYYSNRNNGNAALKAFIVTRIGD
VFMAIGLFILFQQVGTLNIQELLVLAPQKFQVGDFWITLATLMLLGGAVGKSAQLPLQTW
LADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFTLAPDILHLVGIVGGVTLVLAGFAA
LVQTDIKRILAYSTMSQIGYMFLALGVGAWDAAIFHLMTHAFFKALLFLASGAVIVACHH
EQNIFKMGGLWKKLPLAYASFIVGGAALAALPLVTAGFYSKDEILWEAFASGNQNLLYAG
LVGAFMTSLYTFRLIFITFHGEAKTEAHAGHGISHWLPLSVLIILSTFIGALITPPLAGV
LPESAGHAGGAAKHSLEIASGAIAIAGILLAALLFLGKRRFVTAVANSGIGRFLSAWWFA
AWGFDWIYDKLFVKPYLAISHMLRKDPLDQTIGLIPRAAKAGHTALSRSETGQLRWYAAS
MAAGAVLVIGAIVVVAV