Protein Info for PFLU_RS18640 in Pseudomonas fluorescens SBW25-INTG

Annotation: NADH-quinone oxidoreductase subunit NuoG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 904 PF13510: Fer2_4" amino acids 3 to 76 (74 residues), 38.7 bits, see alignment E=2.1e-13 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 651 (648 residues), 719.3 bits, see alignment E=2.5e-220 PF00111: Fer2" amino acids 5 to 72 (68 residues), 30.9 bits, see alignment 5.4e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 127 (40 residues), 56.6 bits, see alignment (E = 3.6e-19) PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 49.4 bits, see alignment 9.3e-17 PF00384: Molybdopterin" amino acids 362 to 634 (273 residues), 66.2 bits, see alignment E=7.1e-22

Best Hits

Swiss-Prot: 94% identical to NUOG_PSEFL: NADH-quinone oxidoreductase subunit G (nuoG) from Pseudomonas fluorescens

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 78% identity to avn:Avin_28490)

MetaCyc: 89% identical to NADH-quinone oxidoreductase subunit G (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JY86 at UniProt or InterPro

Protein Sequence (904 amino acids)

>PFLU_RS18640 NADH-quinone oxidoreductase subunit NuoG (Pseudomonas fluorescens SBW25-INTG)
MATIHVDGKALEVDGADNLLQACLSLGLDIPYFCWHPALGSVGACRQCAVKQYTDENDTR
GRIVMSCMTPATDNTWISIDDEESKAFRASVVEWLMTNHPHDCPVCEEGGHCHLQDMTVM
TGHNERRYRFTKRTHQNQQLGPFISHEMNRCIACYRCVRFYKDYAGGTDLGVFGAHDNVY
FGRVEDGVLESEFSGNLTEVCPTGVFTDKTHSERYNRKWDMQFSPSICHGCSSGCNISPG
ERYGELRRIENRFNGSVNQYFLCDRGRFGYGYVNREDRPRQPLLADGGKLSLDAALDKAA
DLLRGRNIVGIGSPRASLESNYALRELVGAEHFYSGIEAGELERIRLVLQVLNDSPLPVP
NMRDIEDHDAIFVLGEDLTQTAARIALSLRQSVKGKAEDMADAMRVQPWLDAAVKNIGQH
ALNPLFIASLAETKLDDIAEECVHAAPDDLARIGFAVAHALDASAPAVEGLDAEAGELAQ
RIADALLAAKRPLIIAGTSLGSKALIEAAANIAKALKLRDKNGSISLVVPEANSLGLAML
GGESLDAALQAVTDGNADAIVVLENDLYTRTDAAKVDAALNAAKVLIVADHQKTATSERA
DLVLPAATFAEGDGTLVSQEGRAQRFFQVFDPKYMDASILVHEGWRWLHALRATLLNQPI
DWTQLDHVTAAAAASAPQLARIVDAAPSAAFRIKGMKLAREPLRYSGRTAMRADISVHEP
RTPQDNDTAFAFSMEGYSGSVEPRQQVPFAWSPGWNSPQAWNKFQDEVGGHIRAGDPGTR
LIETQGDSLNWFAAVPRPFNPAQGTWQVVPFFHLFGSEENSSKAAPVQERIPEAYVSLAK
SEADRLGVNDGALLSLNVAGQTLRLPLRINEALGAGLVALPKGLAGIPPAIFGKTVDGLQ
EAAQ