Protein Info for PFLU_RS18625 in Pseudomonas fluorescens SBW25-INTG

Annotation: NADH-quinone oxidoreductase subunit C/D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 TIGR01961: NADH (or F420H2) dehydrogenase, subunit C" amino acids 43 to 171 (129 residues), 140.4 bits, see alignment E=4.2e-45 PF00329: Complex1_30kDa" amino acids 44 to 173 (130 residues), 150.2 bits, see alignment E=4.3e-48 TIGR01962: NADH dehydrogenase (quinone), D subunit" amino acids 210 to 594 (385 residues), 567.6 bits, see alignment E=1.2e-174 PF00346: Complex1_49kDa" amino acids 324 to 594 (271 residues), 364.3 bits, see alignment E=3.2e-113

Best Hits

Swiss-Prot: 100% identical to NUOCD_PSEFS: NADH-quinone oxidoreductase subunit C/D (nuoC) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K13378, NADH dehydrogenase I subunit C/D [EC: 1.6.5.3] (inferred from 100% identity to pfs:PFLU3820)

MetaCyc: 89% identical to NADH-quinone oxidoreductase subunit C/D (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JY83 at UniProt or InterPro

Protein Sequence (594 amino acids)

>PFLU_RS18625 NADH-quinone oxidoreductase subunit C/D (Pseudomonas fluorescens SBW25-INTG)
MTTGSALYIPPYKADDQDVVVELNNRFGPDAFTAQATRTGMPVLWVARAKLVEVLTFLRN
LPKPYVMLYDLHGVDERLRTKRQGLPSGADFTVFYHLLSIERNSDVMIKVALSESDLSVP
TVTGIWPNASWYEREVWDMFGIDFPGHPHLTRIMMPPTWEGHPLRKDFPARATEFDPFSL
NLAKQQLEEEAARFRPEDWGMKRSGTNEDYMFLNLGPNHPSAHGAFRIILQLDGEEIVDC
VPDIGYHHRGAEKMAERQSWHSFIPYTDRIDYLGGVMNNLPYVLSVEKLAGIKVPDRVDT
IRIMMAEFFRITSHLLFLGTYIQDVGAMTPVFFTFTDRQRAYKVIEAITGFRLHPAWYRI
GGVAHDLPNGWERLVKEFIDWMPKRLDEYQKAALDNSILKGRTIGVAAYNTKEALEWGVT
GAGLRSTGCDFDLRKARPYSGYENFEFEVPLAANGDAYDRCIVRVEEMRQSLKIIEQCMR
NMPAGPYKADHPLTTPPPKERTLQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSY
YLTSDGGTMSYRTRIRTPSFPHLQQIPSVIKGEMVADLIAYLGSIDFVMADVDR