Protein Info for PFLU_RS17900 in Pseudomonas fluorescens SBW25-INTG

Annotation: acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

No protein families (PFam or TIGRFam), signal peptides, or transmembrane helices were found in this protein.

Best Hits

Swiss-Prot: 45% identical to WCAF_SHIFL: Putative colanic acid biosynthesis acetyltransferase WcaF (wcaF) from Shigella flexneri

KEGG orthology group: K03818, putative colanic acid biosynthesis acetyltransferase WcaF [EC: 2.3.1.-] (inferred from 100% identity to pfs:PFLU3670)

MetaCyc: 45% identical to colanic acid biosynthesis acetyltransferase WcaF (Escherichia coli K-12 substr. MG1655)
2.3.1.-

Predicted SEED Role

"Colanic acid biosynthesis acetyltransferase WcaF (EC 2.3.1.-)" in subsystem Colanic acid biosynthesis (EC 2.3.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JXP1 at UniProt or InterPro

Protein Sequence (189 amino acids)

>PFLU_RS17900 acetyltransferase (Pseudomonas fluorescens SBW25-INTG)
MILQGNDPHRSPSFSLGHRLRRQLWNMVYVLLFRTSPRPFHAWRAFLLRLFGARLGKGVH
VYPRAKVWAPWNLELGDHVGIADGTTLYNMALIRIGRYSVISQGAHLCGGSHDYNSKNFQ
LYAKPIVLGDHVWVCAEAFITLGVSVADGVVVGARTLVIKSIVQPWTVHAGHPARQIGER
TQHLQAVMP