Protein Info for PFLU_RS17795 in Pseudomonas fluorescens SBW25-INTG

Annotation: M20/M25/M40 family metallo-hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 409 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF04389: Peptidase_M28" amino acids 93 to 204 (112 residues), 36.7 bits, see alignment E=7.7e-13 PF01546: Peptidase_M20" amino acids 106 to 404 (299 residues), 112.6 bits, see alignment E=4.9e-36 PF07687: M20_dimer" amino acids 210 to 308 (99 residues), 80.3 bits, see alignment E=2e-26

Best Hits

Swiss-Prot: 38% identical to CBPG_PSES6: Carboxypeptidase G2 (cpg2) from Pseudomonas sp. (strain RS-16)

KEGG orthology group: K01295, glutamate carboxypeptidase [EC: 3.4.17.11] (inferred from 100% identity to pfs:PFLU3649)

Predicted SEED Role

"Glutamate carboxypeptidase (EC 3.4.17.11)" (EC 3.4.17.11)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.17.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JXM0 at UniProt or InterPro

Protein Sequence (409 amino acids)

>PFLU_RS17795 M20/M25/M40 family metallo-hydrolase (Pseudomonas fluorescens SBW25-INTG)
MLFNFSRTLLAATLAVSFAVPAYSAEPHKQIQAHAEQYKAEALKLLERLVNIDSGSGYEP
GLTQVRDIAVDELKQLGFAIEQVPDKAANNSHVVATLKGTGKAKILLMAHMDTVFKEGSA
AERPFHIKDGRAYGPGVMDDKGGIVAGIYALKVLKDQGFKDYAQITFLLDASEETGSDAA
SELIRNTAKAHDVTLNLEPGRPADGLVVWRKGSATAVVEVKGKAAHAGVAPELGRNAAME
VAHQILQLGKLGDEEKKTTINFTVLKAGDRTNVIPDQATAKADVRAALPEEFDRIEKDLV
RVSANKLIPDTEVTTSLKRGLPPMPQTAESDKLVAIAQGIYGELGKKLTIEGSGGAADAS
LSAGVGTPTLDGFGIVGGNIHTPEEYAEVESVVPRVYLLSRMIMELSKR