Protein Info for PFLU_RS17490 in Pseudomonas fluorescens SBW25-INTG

Annotation: glutaminase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to GLSA_PSEFS: Glutaminase (glsA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01425, glutaminase [EC: 3.5.1.2] (inferred from 100% identity to pfs:PFLU3587)

MetaCyc: 61% identical to glutaminase 2 (Escherichia coli K-12 substr. MG1655)
Glutamate synthase (NADPH). [EC: 1.4.1.13, 1.4.1.14, 1.4.7.1, 2.6.1.85, 3.5.1.2, 3.5.1.38, 4.1.3.27, 4.3.2.10, 4.3.3.6, 6.3.4.2, 6.3.5.13, 6.3.5.2, 6.3.5.4, 6.3.5.5, 6.3.5.6, 6.3.5.7]

Predicted SEED Role

"Glutaminase (EC 3.5.1.2)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.2)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
superpathway of aromatic amino acid biosynthesis (18/18 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
superpathway of L-tryptophan biosynthesis (13/13 steps found)
superpathway of purine nucleotide salvage (13/14 steps found)
L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
L-histidine biosynthesis (10/10 steps found)
superpathway of arginine and polyamine biosynthesis (15/17 steps found)
L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
L-citrulline biosynthesis (8/8 steps found)
L-tryptophan biosynthesis (6/6 steps found)
UMP biosynthesis I (6/6 steps found)
UMP biosynthesis II (6/6 steps found)
superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
superpathway of L-citrulline metabolism (10/12 steps found)
superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
superpathway of pyrimidine nucleobases salvage (4/4 steps found)
L-glutamate and L-glutamine biosynthesis (6/7 steps found)
L-citrulline degradation (3/3 steps found)
UTP and CTP de novo biosynthesis (3/3 steps found)
ammonia assimilation cycle III (3/3 steps found)
superpathway of ammonia assimilation (plants) (3/3 steps found)
superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
UMP biosynthesis III (5/6 steps found)
4-aminobenzoate biosynthesis I (2/2 steps found)
L-glutamate biosynthesis I (2/2 steps found)
ammonia assimilation cycle I (2/2 steps found)
ammonia assimilation cycle II (2/2 steps found)
L-asparagine biosynthesis I (1/1 steps found)
L-asparagine degradation I (1/1 steps found)
L-glutamate biosynthesis IV (1/1 steps found)
L-glutamate biosynthesis V (1/1 steps found)
L-glutamine degradation I (1/1 steps found)
L-glutamine degradation II (1/1 steps found)
nitrogen remobilization from senescing leaves (6/8 steps found)
GABA shunt II (3/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
UTP and CTP dephosphorylation I (5/7 steps found)
L-asparagine degradation III (mammalian) (2/3 steps found)
peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
superpathway of chorismate metabolism (42/59 steps found)
superpathway of L-asparagine biosynthesis (1/2 steps found)
UTP and CTP dephosphorylation II (1/3 steps found)
superpathway of pyrimidine ribonucleosides salvage (6/10 steps found)
4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
acridone alkaloid biosynthesis (1/4 steps found)
L-arginine biosynthesis IV (archaea) (4/9 steps found)
superpathway of candicidin biosynthesis (4/11 steps found)
allantoin degradation IV (anaerobic) (2/9 steps found)
superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
chloramphenicol biosynthesis (1/9 steps found)
guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14, 1.4.7.1, 2.6.1.85, 3.5.1.38, 4.1.3.27, 6.3.4.2, 6.3.5.2, 6.3.5.4, 6.3.5.5, 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14 or 1.4.7.1 or 2.6.1.85 or 3.5.1.2 or 3.5.1.38 or 4.1.3.27 or 4.3.2.10 or 4.3.3.6 or 6.3.4.2 or 6.3.5.13 or 6.3.5.2 or 6.3.5.4 or 6.3.5.5 or 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JXF9 at UniProt or InterPro

Protein Sequence (302 amino acids)

>PFLU_RS17490 glutaminase B (Pseudomonas fluorescens SBW25-INTG)
MQAMLSSILDEVRPLIGLGKVADYIPALADVPANQLGIAVYGNDGSAYRAGDADTLFSVQ
SISKVFSLVQAIDHGGETIWERLGHEPSGQPFNSMVQLEFERGRPRNPFINAGALVICDI
NQSRFAVPILSMRDFVRRLSGNPHILVNSVVAESEAQHGARNAAMAYLMKSFGNFHNDVD
AVLHSYFNYCALQMSCVDLAKAFSFLANEGTSAHSGEQILTARQTRQVNSIMATSGLYDE
AGNFAYRVGLPGKSGVGGGIVAVVPGQFTVCVWSPELNAAGNSLAGMKALELLSERIGWS
VF