Protein Info for PFLU_RS17300 in Pseudomonas fluorescens SBW25-INTG

Annotation: type VI secretion system protein TssA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 TIGR03362: type VI secretion-associated protein, VC_A0119 family" amino acids 13 to 483 (471 residues), 379.6 bits, see alignment E=1.4e-117 PF06812: ImpA_N" amino acids 21 to 127 (107 residues), 89.2 bits, see alignment E=2.1e-29 PF16989: T6SS_VasJ" amino acids 228 to 482 (255 residues), 232.9 bits, see alignment E=4.4e-73

Best Hits

KEGG orthology group: K11910, type VI secretion system protein VasJ (inferred from 100% identity to pfs:PFLU3549)

Predicted SEED Role

"Uncharacterized protein ImpA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7U9 at UniProt or InterPro

Protein Sequence (517 amino acids)

>PFLU_RS17300 type VI secretion system protein TssA (Pseudomonas fluorescens SBW25-INTG)
MAYSDKLYDYYLELARSPCMPTSFAGSDMRFSSEYEVLEFELAKARSIHGSGQPDWHKVL
EAGEILLRQQSKDLRVAVWLTWALHQRESYPGLLAGLGLLRYFCEHHWSEVHPGKPRTRG
AAFGWLVLRLEPLFTLSLPLQNQQPLFQAVLEHLIRLDELWAEYLGDAAPMLLPIRRQLA
QRLERAAQDDAPVAGLSGVIAHVKQATTQLLKPEARVDNEKDAHRLLRTLQEQARTLCVW
WLRQNATDLRAFRLNRTLTWLALANYPDADNERLTALRGPAPDKLKRYQERFAQGHHADL
VLELEASLAGAMFWFDGLHMLWECLEAMQAEQAMTELEVTFALLLQRLPNLAEFRFHDGA
PFAGPATRDWIALQVTRHLQIPESPALIVDADAEPWEIALQAVATRLRKDGLKAAIHELK
QGMYAARSDRARFYWRLAQARLCVLAGKHELAKIQLEQLDHELQHTGLERWEPELALQVT
QLLYRCCDLLPQNHAVRERKEDTHRRLCLFDLEAVLE