Protein Info for PFLU_RS16820 in Pseudomonas fluorescens SBW25-INTG

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 54 to 78 (25 residues), see Phobius details amino acids 89 to 109 (21 residues), see Phobius details amino acids 111 to 129 (19 residues), see Phobius details amino acids 151 to 172 (22 residues), see Phobius details amino acids 178 to 199 (22 residues), see Phobius details amino acids 222 to 241 (20 residues), see Phobius details amino acids 264 to 282 (19 residues), see Phobius details amino acids 293 to 311 (19 residues), see Phobius details amino acids 317 to 338 (22 residues), see Phobius details amino acids 348 to 371 (24 residues), see Phobius details amino acids 379 to 398 (20 residues), see Phobius details PF07690: MFS_1" amino acids 29 to 272 (244 residues), 87 bits, see alignment E=6.3e-29 amino acids 232 to 398 (167 residues), 53.1 bits, see alignment E=1.2e-18

Best Hits

Swiss-Prot: 59% identical to FSR_ECOLI: Fosmidomycin resistance protein (fsr) from Escherichia coli (strain K12)

KEGG orthology group: K08223, MFS transporter, FSR family, fosmidomycin resistance protein (inferred from 100% identity to pfs:PFLU3446)

MetaCyc: 59% identical to fosmidomycin efflux pump (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-41

Predicted SEED Role

"fosmidomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KCF5 at UniProt or InterPro

Protein Sequence (403 amino acids)

>PFLU_RS16820 MFS transporter (Pseudomonas fluorescens SBW25-INTG)
MSTLTASPAAATTAPQASPLVMRILGACALAHLINDLIQAVLPSIYPMLKANYGLSFTQV
GLITLTFQLTASLLQPWIGYHTDRHPKPWLLPAGMVCTLIGILMLAFVGNFPAILLAAGL
VGVGSSTFHPETSRVARLASGGRYGLAQSTFQVGGNTGSALGPLLAAAIIIPYGQGHIAW
FGLFAVFAILVLYGLSRWYRNHLNLFKLKQGGKATHGLSKGRVTFALVVLAVLVFSKYWY
MTSLTSYFTFYLIEKFQLSVSSSQMYLFLFLGAVAVGTFAGGPIGDKIGRKKVIWFSILG
AAPFTLALPYVDLFWTAVLSVVIGFIIASAFSAIVVFAQELVPGNVGMIAGIFFGLMFGF
SGIGAALLGLLADTHGIEYVYKLCSFLPLVGILTVLLPSTKGI