Protein Info for PFLU_RS16800 in Pseudomonas fluorescens SBW25-INTG

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 35 to 56 (22 residues), see Phobius details amino acids 63 to 82 (20 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 121 to 143 (23 residues), see Phobius details amino acids 149 to 173 (25 residues), see Phobius details amino acids 194 to 215 (22 residues), see Phobius details amino acids 227 to 248 (22 residues), see Phobius details amino acids 255 to 275 (21 residues), see Phobius details amino acids 282 to 306 (25 residues), see Phobius details amino acids 318 to 340 (23 residues), see Phobius details amino acids 347 to 367 (21 residues), see Phobius details PF07690: MFS_1" amino acids 2 to 313 (312 residues), 124 bits, see alignment E=3.3e-40

Best Hits

Swiss-Prot: 62% identical to OPDE_PSEAE: Transcription regulatory protein OpdE (opdE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3442)

MetaCyc: 41% identical to purine ribonucleoside exporter (Escherichia coli K-12 substr. MG1655)
RXN0-18; RXN0-22

Predicted SEED Role

"Arabinose efflux permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KCF1 at UniProt or InterPro

Protein Sequence (386 amino acids)

>PFLU_RS16800 MFS transporter (Pseudomonas fluorescens SBW25-INTG)
MSLAAFALVASEFLPVSLLTPLATDLHITEGQAGQGISVSGLFALLTSLVIAAVAARVER
KRLLLSLTLLMIISGTVVAFAPNYSVFMLGRALIGVAIGGFWSLSAATAMRLVPAAQVPR
ALAIVNGGNALATVIAAPLGSFVGGLIGWRGAFFCVVPVAVLAAAWLMLSLPSIKAQGAA
KNGRLLGLMKRKPVALGMLAVSVFFMGQFMLFTYLRPFLEAVTQVSVSRLSLILLVLGLA
GLVGTLLIERLLKKSVFLTLVIIPLVMAGIALALVKVGSSMVMTTFLLGLWGLVATAAPV
GWWTWLSTTLPDDAEAGGGLMVAIIQLAIASGAIVGGLVFDWSGYQATFQLSAAVLGAAA
VLAWLAARASGYRALSMICPPSGVRL