Protein Info for PFLU_RS16425 in Pseudomonas fluorescens SBW25-INTG

Annotation: malto-oligosyltrehalose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 916 TIGR02401: malto-oligosyltrehalose synthase" amino acids 7 to 908 (902 residues), 947.9 bits, see alignment E=2.7e-289 PF00128: Alpha-amylase" amino acids 28 to 96 (69 residues), 43.2 bits, see alignment 2e-15

Best Hits

KEGG orthology group: K06044, (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase [EC: 5.4.99.15] (inferred from 100% identity to pfs:PFLU3367)

Predicted SEED Role

"Malto-oligosyltrehalose synthase (EC 5.4.99.15)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 5.4.99.15)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KD20 at UniProt or InterPro

Protein Sequence (916 amino acids)

>PFLU_RS16425 malto-oligosyltrehalose synthase (Pseudomonas fluorescens SBW25-INTG)
MNALPLRATQRLQFHKGFTLDDAVPLVPYFAQLGISHLYASPLLSARAGSMHGYDVVDPT
SVNPELGGEPALRRLVAALREHKMGLILDIVSNHMAVGGADNPWWLDLLEWGRLSPYSEF
FDIQWHSPDPLLKGQLLMPFLGSDYGEALQAGTLTLQFDAPHGAFYVEHYEHRFPICPKD
YAAILGTEAPLKPLAERFTALAHQEDAYHEAAWLKQALAERATEVLPAIEQRLTTFDGRQ
PEGFKRLHQLLEQQAYRLASWRTAADDINWRRFFDVNELGGLRVERTAVFEATHGKIFEL
ISEGLVDGLRIDHIDGLADPRGYCRKLRRRVDSLVPERHLPIFVEKILGEGETLREDWQV
DGTTGYEFMNQLSLLQHQPDGFEPLAELWTRHSERPAAFIEEARLARQQILNGSLGGDFE
SVAQALLQVARDDVMTRDLTLGAIRRALQELIVHFPVYRTYISARGRSAADDKVFQQAMD
GARTTLGEGDWPVLEHLENWLGGQPWRNRPVGRERKILKHACVRFQQLTSPAAAKAVEDT
AFYRSAVLLSRNDVGFSTEQFSAPLADFHAVNQHRLQTFPDNLLATATHDHKRGEDTRAR
LAVLSECAPWYVEQVEHWRSLAAPLRVDANTPSAGDELILYQALLGSWPLDQDADFEGYQ
QRLWQWQQKALREAKLQSSWSAPNEAYEQGVEAFLSRLLLTDEGRELRTAIGNAAQVIAP
AGALNGLAQSLLRITVPGVPDLYQGDEFWDFSLVDPDNRRPVDFNARQQALDTPPDLGEL
LFNWRDGRIKQALIAQVLGLRKAHPELFRSGAYTPLEVVGTHAERVVAFAREYQGKYVLV
VVPRWSHALLENGVHPQVNARVWGDTRVKLPFAATTQNWKGLFHTGAVTPDKELLISTAL
GDFPVNVFINPDDQES