Protein Info for PFLU_RS16355 in Pseudomonas fluorescens SBW25-INTG

Annotation: acylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 761 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF01804: Penicil_amidase" amino acids 34 to 750 (717 residues), 467.4 bits, see alignment E=6.6e-144

Best Hits

Swiss-Prot: 70% identical to PVDQ_PSEPF: Acyl-homoserine lactone acylase PvdQ (pvdQ) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K07116, (no description) (inferred from 100% identity to pfs:PFLU3353)

MetaCyc: 56% identical to acyl-homoserine lactone deacylase precursor (Pseudomonas aeruginosa)

Predicted SEED Role

"penicillin amidase family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KD06 at UniProt or InterPro

Protein Sequence (761 amino acids)

>PFLU_RS16355 acylase (Pseudomonas fluorescens SBW25-INTG)
MIISNGLSRVCVAGVLLGLSLAATAREQVTQASADIRRTSYGVPHIRANDERGLGFGIGY
AYAQDNLCLLANEVVTVNGQRARFFGPEQATLEERNNLASDVFFNWLNPPDAVAAFWKAQ
SPQIQQRIEGYVAGYNRYLKEQGAPAQCQAAWVRPLVAEDLVKLTRRLLVEGGVGQFAEA
LVGATPPQATASVTSSAKAFELAAANQQRFAFDRGSNAVAVGRDRSFNGRGMLLANPHFP
WVGGMRFYQMHLTIPGQLDVMGAALPGLPVINIGFNQHVAWTHTVDTSKHFTLYRLTLDP
KDATRYMLDGKSVPLDKTTVNVQVKQADGSLKDQSHTVYSSPFGPVVQWPGKLDWDSHYA
FSLRDANLGNDRVLQQWYAMNRAASLNELKTSVHTLQGIPWVNTLAADDQGQSLYMNQSV
VPNVSAAKLAQCSDPRAGLQLILLDGAHSACAWDIDPRAAQPGIFAADQLPQLQRTDYVQ
HSNDSAWLANPKAPLTGFSPVISQDHIGLGPRARFALQRLQSLQKPISVTDLQNMVMDNE
VYLAGQVMPDLLDFCAKHLGADANVLQPLCSSLKNWDQRANLDSGIGLVHFINLVQQLQQ
IPDAWRVAFDPAQPLTTPRGLAIDRAPVAKALREAMLASSADVEKLGLTADSTWGDIQVS
GQTPIHGGPQELGIYNAMQTVPRADGKREVVSGSSYLQIVTFDDQGPHAQGVLAFSESTN
PASAHSNDQTQAFSQKKLSPLPFTEAQIKADPQYQQLRIKE