Protein Info for PFLU_RS16205 in Pseudomonas fluorescens SBW25-INTG

Annotation: dihydrodipicolinate synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00701: DHDPS
  • amino acids 6 to 236 (231 residues), 90.9 bits, E=3.2e-30

Best Hits

KEGG orthology group: K01714, dihydrodipicolinate synthase [EC: 4.2.1.52] (inferred from 100% identity to pfs:PFLU3325)

MetaCyc: 88% identical to Delta1-pyrroline-4-hydroxy-2-carboxylate deaminase subunit (Pseudomonas putida KT2442)
1-pyrroline-4-hydroxy-2-carboxylate deaminase. [EC: 3.5.4.22]

Predicted SEED Role

"1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.5.4.22)

MetaCyc Pathways

• trans-4-hydroxy-L-proline degradation II (4/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.52

Use Curated BLAST to search for 3.5.4.22 or 4.2.1.52

Sequence Analysis Tools

See C3KCC5 at UniProt or InterPro

Protein Sequence (315 amino acids)

>PFLU_RS16205 dihydrodipicolinate synthase family protein (Pseudomonas fluorescens SBW25-INTG)
MSDNIFTGCIPALMTPCTPARKPDFDALVAKGRELIDIGMSAVVYCGSMGDWPLLTEAER
QEGVARLVAAGVPTIVGTGAVNSREAVAHAAHAAKVGAHGLMVIPRVLSRGASATAQKAH
FSAILKAAPNLPAVIYNSPYYGFATRAELFFELRREHPNLIGFKEFGGGADLRYAAEHIT
SQDDNVTLMVGVDTQVVHGFVNCNATGAITGIGNALPREVLQLVALSKKAAKGDAKARRQ
ARELEAALAVLSSFDEGCDLVLYYKHLMVLNGDKEYALHFNETDKLSDAQRRYAETQYLL
FRQWYAHWSAEQHVA