Protein Info for PFLU_RS16200 in Pseudomonas fluorescens SBW25-INTG

Annotation: 4-hydroxyproline epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05544: Pro_racemase
  • amino acids 6 to 308 (303 residues), 334.5 bits, E=3.2e-104

Best Hits

Swiss-Prot: 80% identical to 4HYPE_PSEPK: 4-hydroxyproline 2-epimerase (proR) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K12658, 4-hydroxyproline epimerase [EC: 5.1.1.8] (inferred from 100% identity to pfs:PFLU3324)

MetaCyc: 80% identical to hydroxyproline 2-epimerase subunit (Pseudomonas putida)
4-hydroxyproline epimerase. [EC: 5.1.1.8]

Predicted SEED Role

"4-hydroxyproline epimerase (EC 5.1.1.8)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 5.1.1.8)

MetaCyc Pathways

• trans-4-hydroxy-L-proline degradation II (4/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.8

Sequence Analysis Tools

See C3KCC4 at UniProt or InterPro

Protein Sequence (308 amino acids)

>PFLU_RS16200 4-hydroxyproline epimerase (Pseudomonas fluorescens SBW25-INTG)
MKRLHVIDSHTGGEPTRLVMNGFPKLIGNTLAEQLNNLRSEHDKWRRACILEPRGNDVLV
GALYCEPVTPGAICGVIFFNNAGYLGMCGHGTIGLVASLHHVGRIAPGMHRIDTPVGLVE
ATLHEDGAVTLGNVPAYRHRRQVPVEVPGHGVVLGDVAWGGNWFFLVSEHGQRLQMDNVD
ALTDYTWAMLKALEDQGIHGADGALIDHIELFADDDHADSRNFVMCPGKAYDRSPCGTGT
SAKLACLAADGTLAPGATWTQASITGSQFEGRYEWDGERVRPFITGRAYMTADSTLLIDE
QDPFAWGI