Protein Info for PFLU_RS16095 in Pseudomonas fluorescens SBW25-INTG

Annotation: 3-(3-hydroxy-phenyl)propionate transporter MhpT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 49 to 69 (21 residues), see Phobius details amino acids 77 to 95 (19 residues), see Phobius details amino acids 101 to 124 (24 residues), see Phobius details amino acids 135 to 157 (23 residues), see Phobius details amino acids 163 to 183 (21 residues), see Phobius details amino acids 217 to 237 (21 residues), see Phobius details amino acids 253 to 273 (21 residues), see Phobius details amino acids 280 to 300 (21 residues), see Phobius details amino acids 306 to 326 (21 residues), see Phobius details amino acids 337 to 358 (22 residues), see Phobius details amino acids 368 to 390 (23 residues), see Phobius details PF07690: MFS_1" amino acids 16 to 247 (232 residues), 117.9 bits, see alignment E=7.4e-38 amino acids 219 to 395 (177 residues), 47.8 bits, see alignment E=1.5e-16 PF06779: MFS_4" amino acids 36 to 384 (349 residues), 31.9 bits, see alignment E=1.4e-11 PF00083: Sugar_tr" amino acids 45 to 200 (156 residues), 67.1 bits, see alignment E=2.3e-22

Best Hits

Swiss-Prot: 52% identical to MHPT_ECOLI: 3-(3-hydroxy-phenyl)propionate transporter (mhpT) from Escherichia coli (strain K12)

KEGG orthology group: K05819, MFS transporter, AAHS family, 3-hydroxyphenylpropionic acid transporter (inferred from 100% identity to pfs:PFLU3303)

MetaCyc: 52% identical to 3-hydroxyphenylpropionate/3-hydroxycinnamate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-61; TRANS-RXN0-457

Predicted SEED Role

"Putative hydroxycinnamate transporter" in subsystem Phenylpropanoid compound degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KCA3 at UniProt or InterPro

Protein Sequence (402 amino acids)

>PFLU_RS16095 3-(3-hydroxy-phenyl)propionate transporter MhpT (Pseudomonas fluorescens SBW25-INTG)
MDSPSRRSTLTIALCFIVALIEGFDLQAAGTAAAGLRQTFALDPKMLGWVFSVGIIGLLP
GAFFGGWVADRIGRKKILVMAVLLFGVFSLSTAFVDSYSGLLLVRFLTGLGLGAALPNLI
ALCAEAVSEHKRGTAISVMYCGVPLGGALAAVVAMFSSEHWQTTFIIGGLAPLLAVPLMI
LLLPESSAFRQQRDTPPGARPSTSQALFGEGRARTTLALWLSYFFTLTVMYMLLNWLPSL
LLEQGFSKPQAGMVQMLFNIGGALGSLLGGVLLDRCNAVKVVLCVYAGLLAALAGVGLSV
GIVPMAIAGFAAGLFVMAAQLVLYASAPPSYPTSVRATGVGAAVAVGRLGSVAGPLAAGQ
LLAAGAGTAGVLLATSPGVVIAALTIISVMVRAAPPRTCAAN