Protein Info for PFLU_RS16070 in Pseudomonas fluorescens SBW25-INTG

Annotation: feruloyl-CoA synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 624 PF00501: AMP-binding" amino acids 55 to 434 (380 residues), 159.7 bits, see alignment E=5e-51

Best Hits

KEGG orthology group: K12508, feruloyl-CoA synthase [EC: 6.2.1.34] (inferred from 100% identity to pfs:PFLU3298)

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KC98 at UniProt or InterPro

Protein Sequence (624 amino acids)

>PFLU_RS16070 feruloyl-CoA synthase (Pseudomonas fluorescens SBW25-INTG)
MSSEFRPQPQPTPRYREVSIGHAPVQVTEEQGVLHMRSLEPLGELPARLLDRLVHWAQVR
PEQTFIAARQAGGDWRRVSYREMLDSVRAIAQGLLRYGLSADKPLALLSGNDIEHLQVAL
GAMYAGIPYCPVSPAYSLLSQDFAKLRHVCNLLQPGLVFVSDASAYQRAIDAVLPVETPL
ISVRGYAPGRTQASFASLLQTPGGAEAEAAFKATGPDSIAKFLFTSGSTKLPKAVVTTQR
MLCANQQMLLQTFPVFGEEPPVLVDWLPWNHTFGGSHNVGIVLYNGGTFYLDDGKPTVQG
FAETLRNLKDVSPTAYLTVPKGWEELVNALEQDAELRERFFARMKLFFFAAAGLSQSIWD
RLDRVAERHCGERIRMMAGLGMTEAAPSCTFTTGPLSMAGYIGLPAPGCEVRLVPLDGKF
EGRFRGPHIMPGYWRAPEQTAEVFDELGFYCSGDAIKLADPQQPQLGLMFDGRIAEDFKL
SSGVFVSVGPLRNRAVLEGSPYVQDLVIAAPDRECLGALVFPRLYECRRLAGLSTDASDA
EVLASAPVRAWFGDWLARLNREATGNASRLEWIAVLDEPASIDRGEITDKGSINQRAVLQ
WRAARVEALYRGEDPTILRAGPKP