Protein Info for PFLU_RS16025 in Pseudomonas fluorescens SBW25-INTG

Annotation: flavin-dependent monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 transmembrane" amino acids 67 to 81 (15 residues), see Phobius details PF08028: Acyl-CoA_dh_2" amino acids 236 to 367 (132 residues), 92.9 bits, see alignment E=2.1e-30

Best Hits

Swiss-Prot: 71% identical to HPAH_ACIBA: p-hydroxyphenylacetate 3-hydroxylase, oxygenase component (C2-hpah) from Acinetobacter baumannii

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3290)

Predicted SEED Role

"P-hydroxyphenylacetate hydroxylase C2:oxygenase component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KC90 at UniProt or InterPro

Protein Sequence (389 amino acids)

>PFLU_RS16025 flavin-dependent monooxygenase (Pseudomonas fluorescens SBW25-INTG)
MKKPNPLLEDLTSILPTIAANAFMAEQDRKVPAENIALLKSIGLHRAFQPKAFGGMELSL
PQFADCIALLAGACASTAWAMSLLCTHSHQLALFSAQLQQEVWGTDPNATASSSIAPFGR
TEEVDGGVLFSGEMGWSSGCDHAEWAIVGFRRNNAQGTQDYCFAVLPRSDYQIRDDWFAA
GMKGSGTKTLIIDKARVPEHRIQKAKDMMEGKSAGFGLYPDSKIFYSPYRPYFASGFSTV
SLGVAERMLEVFREKTKTRVRAYTGAAVGAATPALMRLAESTHQVAAARAFLEKTWNEHA
EHSEQQRYPSRETLAFWRTNQAYATKMCIQAVDRLFEAAGGNAWFEHNEMQRLFRDSHMT
GAHAYTDYDVCAQILGRELMGLEPDPSMI